Drake H L, Akagi J M
J Bacteriol. 1977 Oct;132(1):139-43. doi: 10.1128/jb.132.1.139-143.1977.
Bisulfite reductase, purified from Desulfovibrio vulgaris, was coupled with the pyruvate phosphoroclastic reaction. Moderate to low reducing conditions resulted in the formation of trithionate; however, when the concentration of reductant was high, a mixture of trithionate and thiosulfate was formed. Sulfide was also a detectable product, but only when the concentration of bisulfite was low. Flavodoxin mediated native coupling between bisulfite reductase and the phosphoroclastic reaction. A model for bisulfite reductase activity is proposed.
从普通脱硫弧菌中纯化得到的亚硫酸氢盐还原酶与丙酮酸磷酸裂解反应偶联。适度到低度的还原条件导致连三硫酸盐的形成;然而,当还原剂浓度较高时,会形成连三硫酸盐和硫代硫酸盐的混合物。硫化物也是可检测到的产物,但仅在亚硫酸氢盐浓度较低时出现。黄素氧还蛋白介导了亚硫酸氢盐还原酶与磷酸裂解反应之间的天然偶联。提出了亚硫酸氢盐还原酶活性的模型。