Department of Chemistry and Biochemistry and International Forensic Research Institute, Florida International University, Miami, FL 33199, USA.
Electrophoresis. 2013 Jun;34(11):1539-47. doi: 10.1002/elps.201200570.
In this paper, a rapid thermal cycling procedure is combined with a direct amplification from a paper punch, permitting a high-speed amplification of a 7-locus multiplex that requires no extraction step. When coupled with a short 1.8 cm microfluidic electrophoresis system, the entire procedure from paper punch to genotype can be completed in under 25 min. The paper describes selection and optimization of enzyme, direct amplification conditions, the reproducibility of the procedure, and concordance with standard forensic genotyping methods. The procedure utilizes a small high-speed thermal cycler and microfluidic device along with a small laptop and is highly portable. Overall, this technique should provide a useful and reliable procedure for rapid determination of identity of individuals retained at checkpoints as well as a quick method for preliminary identification of individuals at remote locations following mass disasters.
在本文中，我们将快速热循环程序与直接从纸冲孔中进行的直接扩增相结合，实现了无需提取步骤的 7 个基因座多重扩增的高速扩增。当与短的 1.8 厘米微流控电泳系统结合使用时，从纸冲孔到基因型的整个过程可以在 25 分钟内完成。本文描述了酶的选择和优化、直接扩增条件、该程序的可重复性以及与标准法医基因分型方法的一致性。该程序利用小型高速热循环器和微流控装置以及小型笔记本电脑，具有高度便携性。总的来说，该技术应该为快速确定检查站留存人员的身份以及在大规模灾难后快速初步识别偏远地区人员提供有用且可靠的方法。
