Shin Min Ki, Jeon Seon Min, Koo Yong Eui
Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, 08826 Republic of Korea.
Food Safety Risk Assessment Department, National Institute of Food and Drug Safety Evaluation, Cheongju, 28159 Republic of Korea.
Food Sci Biotechnol. 2023 Feb 1;32(9):1-7. doi: 10.1007/s10068-023-01258-5.
Genetically modified (GM) potatoes having resistance to insects and viral diseases, low reducing sugar contents, and black spots for high quality continue to be developed. However, no GM potato has been approved as food or feed in the Republic of Korea as the country adheres to a zero-tolerance policy to unauthorized genetically modified organisms (GMOs). When the self-sufficiency rate is low, a detection method to assess GMOs in crops or other products is necessary. Therefore, a rapid method for two GM potato events (SPS-Y9 and EH92-527-1) using an ultra-fast PCR (UF-PCR) system has been developed, and its specificity, sensitivity, and applicability were demonstrated. UF-PCR can decrease the runtime of PCR by more than half of that needed in conventional methods. However, UF-PCR is not a common method for GMO analysis. This rapid detection method may be useful for GMO analyses in field conditions.
具有抗虫和抗病毒病能力、低还原糖含量以及高质量无黑斑的转基因马铃薯仍在持续研发中。然而，由于韩国对未经授权的转基因生物（GMO）坚持零容忍政策，尚无转基因马铃薯在韩国被批准用于食品或饲料。在自给率较低的情况下，需要一种检测方法来评估作物或其他产品中的转基因生物。因此，已开发出一种使用超快速PCR（UF-PCR）系统检测两种转基因马铃薯事件（SPS-Y9和EH92-527-1）的快速方法，并证明了其特异性、灵敏度和适用性。UF-PCR可将PCR运行时间缩短至传统方法所需时间的一半以上。然而，UF-PCR并非转基因生物分析的常用方法。这种快速检测方法可能在田间条件下的转基因生物分析中有用。
