Yamada H, Komiyama K, Kiyohara H, Cyong J C, Hirakawa Y, Otsuka Y
Oriental Medicine Research Center, Kitasato Institute, Tokyo, Japan.
Planta Med. 1990 Apr;56(2):182-6. doi: 10.1055/s-2006-960921.
The polysaccharide fraction from the root of Angelica acutiloba Kitagawa showed a potent antitumor activity against ascitic form of Sarcoma-180, IMC carcinoma, and Meth A fibrosarcoma as well as the solid form of MM-46 tumor. An active polysaccharide, AR-4E-2, was purified by precipitation with cetyl-trimethylammonium bromide, anion-exchange chromatography on DEAE-Sepharose, and gel filtration on Sepharose CL-4B from the polysaccharide fraction. An active polysaccharide fraction showed a weak anti-complementary activity. AR-4E-2 was composed of arabinose, galactose, and rhamnose in the molar ratios of 3.3:1.0:0.7, and also contained 14.5% galacturonic acid and 3.2% protein. Methylation analysis and base-catalysed beta-elimination studies suggested that AR-4E-2 contained a rhamnogalacturonan moiety in which 2,4-di-substituted rhamnose residues were attached to 4-substituted galacturonic acid through position 2 of rhamnose. AR-4E-2 also contained highly branched 3,5-arabinan and (1----4)-galactan.
北川当归根中的多糖组分对腹水型肉瘤-180、IMC癌、Meth A纤维肉瘤以及实体型MM-46肿瘤均显示出强大的抗肿瘤活性。通过用十六烷基三甲基溴化铵沉淀、在DEAE-琼脂糖上进行阴离子交换色谱以及在琼脂糖CL-4B上进行凝胶过滤，从多糖组分中纯化出一种活性多糖AR-4E-2。一种活性多糖组分显示出较弱的抗补体活性。AR-4E-2由阿拉伯糖、半乳糖和鼠李糖以摩尔比3.3:1.0:0.7组成，还含有14.5%的半乳糖醛酸和3.2%的蛋白质。甲基化分析和碱催化的β-消除研究表明，AR-4E-2含有一个鼠李半乳糖醛酸聚糖部分，其中2,4-二取代的鼠李糖残基通过鼠李糖的2位连接到4-取代的半乳糖醛酸上。AR-4E-2还含有高度分支的3,5-阿拉伯聚糖和(1→4)-半乳聚糖。
