用于增强人骨髓间充质干细胞心脏分化的聚（己内酯）-碳纳米管复合支架。
Poly(ε-caprolactone)-carbon nanotube composite scaffolds for enhanced cardiac differentiation of human mesenchymal stem cells.
机构信息
Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA.
出版信息
Nanomedicine (Lond). 2013 Nov;8(11):1763-76. doi: 10.2217/nnm.12.204. Epub 2013 Mar 27.
Abstract
AIM
To evaluate the efficacy of electrically conductive, biocompatible composite scaffolds in modulating the cardiomyogenic differentiation of human mesenchymal stem cells (hMSCs).
MATERIALS & METHODS: Electrospun scaffolds of poly(ε-caprolactone) with or without carbon nanotubes were developed to promote the in vitro cardiac differentiation of hMSCs.
RESULTS
Results indicate that hMSC differentiation can be enhanced by either culturing in electrically conductive, carbon nanotube-containing composite scaffolds without electrical stimulation in the presence of 5-azacytidine, or extrinsic electrical stimulation in nonconductive poly(ε-caprolactone) scaffolds without carbon nanotube and azacytidine.
CONCLUSION
This study suggests a first step towards improving hMSC cardiomyogenic differentiation for local delivery into the infarcted myocardium.
摘要
目的
评估导电、生物相容的复合支架在调节人心肌干细胞（hMSCs）向心肌细胞分化中的功效。
材料与方法
制备聚己内酯的电纺支架，其中包含或不包含碳纳米管，以促进 hMSCs 的体外心脏分化。
结果
结果表明，hMSC 分化可以通过在含有 5-氮杂胞苷的情况下在导电、含碳纳米管的复合支架中培养而增强，或者在没有碳纳米管和氮杂胞苷的情况下在外源性电刺激下在非导电的聚己内酯支架中增强。
结论
本研究为局部递送至梗死心肌的 hMSC 心肌生成分化的改善迈出了第一步。
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