Fehlmann M, Starita-Geribaldi M, Thiebaut C, Sudaka P
Arch Int Physiol Biochim. 1978 Aug;86(3):601-12. doi: 10.3109/13813457809055928.
Dog enterocyte brush border proteins have been studied after a 75% proximal resection of the small bowel. This study was carried on microvillar membrane preparations purified from ileal mucosa sampled before and after regeneration on neighbouring intestinal segments, each animal acting as its own control. After six weeks of regeneration a statistically significant decrease of the following enzyme specific activities was observed: lactase, cellobiase, maltase, sucrase, palatinase, dextranase, trehalase, alkaline phosphatase, aminopeptidase and gamma-glutamyl transferase. Analysis of brush border proteins by polyacrylamide gel electrophoresis in presence of sodium dodecyl sulphate have shown after regeneration a decreased rate for the proteins with a molecular weight higher than 100,000 daltons. Modifications of electrophoretic patterns seem to be related to the specific activity decreases observed for brush border enzymes after regeneration, since the molecular weight of these enzymes were found between 116,000 and 285,000 daltons, after gel filtration.
在对小肠进行75%的近端切除术后，对犬小肠上皮细胞刷状缘蛋白进行了研究。本研究是在从相邻肠段再生前后取样的回肠黏膜中纯化得到的微绒毛膜制剂上进行的，每只动物都作为自身对照。再生六周后，观察到以下酶的比活性有统计学意义的下降：乳糖酶、纤维二糖酶、麦芽糖酶、蔗糖酶、帕拉金酶、右旋糖酐酶、海藻糖酶、碱性磷酸酶、氨肽酶和γ-谷氨酰转移酶。在十二烷基硫酸钠存在的情况下，通过聚丙烯酰胺凝胶电泳对刷状缘蛋白进行分析，结果显示再生后分子量高于100,000道尔顿的蛋白质的电泳迁移率降低。电泳图谱的改变似乎与再生后刷状缘酶比活性的降低有关，因为在凝胶过滤后发现这些酶的分子量在116,000至285,000道尔顿之间。
