Department of Industrial and Information Engineering, Second University of Naples, 81031, Aversa, Italy.
J Biomed Mater Res A. 2014 Feb;102(2):392-9. doi: 10.1002/jbm.a.34721. Epub 2013 May 14.
When surface-reactive (bioactive) coatings are applied to medical implants by means of the sol-gel dip-coating technique, the biological proprieties of the surface of the implant can be locally modified to match the properties of the surrounding tissues to provide a firm fixation of the implant. The aim of this study has been to synthesize, via sol-gel, organoinorganic nanoporous materials and to dip-coat a substrate to use in dental applications. Different systems have been prepared consisting of an inorganic zirconium-based matrix, in which a biodegradable polymer, the poly-ε-caprolactone was incorporated in different percentages. The materials synthesized by the sol-gel process, before gelation, when they were still in sol phase, have been used to coat a titanium grade 4 (Ti-4) substrate to change its surface biological properties. Thin films have been obtained by means of the dip-coating technique. A microstructural analysis of the obtained coatings was performed using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy. The biological proprieties have been investigated by means of tests in vitro. The bone-bonding capability of the nanocomposite films has been evaluated by examining the appearance of apatite on their surface when plunged in a simulated body fluid (SBF) with ion concentrations nearly equal to those of human blood plasma. The examination of apatite formation on the nanocomposites, after immersion in SBF, has been carried out by SEM equipped with energy-dispersive X-ray spectroscopy. To evaluate cells-materials interaction, human osteosarcoma cell line (Saos-2) has been seeded on specimens and cell vitality evaluated by WST-8 assay.
当通过溶胶-凝胶浸涂技术将表面反应（生物活性）涂层施加到医疗植入物上时，可以局部修改植入物表面的生物特性，以匹配周围组织的特性，从而提供植入物的牢固固定。本研究的目的是通过溶胶-凝胶合成有机-无机纳米多孔材料，并浸涂基底以用于牙科应用。已经制备了不同的系统，这些系统由无机锆基基质组成，其中包含可生物降解的聚合物聚己内酯（PCL），以不同的百分比掺入。溶胶-凝胶工艺合成的材料在凝胶化之前，当其仍处于溶胶相时，已被用于涂覆钛 4 级（Ti-4）基底以改变其表面生物特性。通过浸涂技术获得了薄膜。使用扫描电子显微镜（SEM）和傅里叶变换红外光谱对获得的涂层进行了微观结构分析。通过体外试验研究了生物性能。通过检查将纳米复合材料薄膜浸入具有与人类血浆离子浓度几乎相等的模拟体液（SBF）中时其表面上是否出现磷灰石，评估了纳米复合材料的骨结合能力。在浸入 SBF 后，通过 SEM 配备能量色散 X 射线光谱对磷灰石在纳米复合材料上的形成进行了检查。为了评估细胞-材料相互作用，将人骨肉瘤细胞系（Saos-2）接种到标本上，并通过 WST-8 测定评估细胞活力。
