Sagawa J, Miyagi T, Tsuiki S
Research Institute for Tuberculosis and Cancer, Tohoku University, Sendai.
Tohoku J Exp Med. 1990 Feb;160(2):149-56. doi: 10.1620/tjem.160.149.
Intraperitoneal treatment of rat peritoneal macrophages with OK-432 results in more than 9-fold increase in the activity of ganglioside sialidase, which seems to coincide with the appearance of a cell surface antigen, asialo-GM1. The results of subsequent studies suggest that the ganglioside sialidase is located in the plasma membrane, where the enzyme may be responsible for the formation of asialo-GM1 from GM1. In the macrophages activated with OK-432, sialidase activity toward 4-methylumbelliferyl-N-acetylneuraminic acid (4MU-NeuAc) is also increased. It appears that the 4MU-NeuAc sialidase is intralysosomal and is increased together with other acid hydrolases present in the lysosomes.
用OK-432对大鼠腹膜巨噬细胞进行腹腔内处理，会导致神经节苷脂唾液酸酶的活性增加9倍以上，这似乎与细胞表面抗原脱唾液酸GM1的出现相一致。后续研究结果表明，神经节苷脂唾液酸酶位于质膜中，该酶可能负责从GM1形成脱唾液酸GM1。在用OK-432激活的巨噬细胞中，对4-甲基伞形酮基-N-乙酰神经氨酸（4MU-NeuAc）的唾液酸酶活性也会增加。似乎4MU-NeuAc唾液酸酶存在于溶酶体内，并且与溶酶体中存在的其他酸性水解酶一起增加。
