Experimental Atmospheric Pollution Laboratory (LPAE), Department of Pathology, São Paulo Medical School, University of São Paulo, Av. Dr. Arnaldo 455/Room 1155, 01246-903 São Paulo, SP, Brazil.
Evid Based Complement Alternat Med. 2013;2013:549879. doi: 10.1155/2013/549879. Epub 2013 Feb 27.
Anacardic acids from cashew nut shell liquid, a Brazilian natural substance, have antimicrobial and antioxidant activities and modulate immune responses and angiogenesis. As inflammatory lung diseases have been correlated to environmental pollutants exposure and no reports addressing the effects of dietary supplementation with anacardic acids on lung inflammation in vivo have been evidenced, we investigated the effects of supplementation with anacardic acids in a model of diesel exhaust particle- (DEP-) induced lung inflammation. BALB/c mice received an intranasal instillation of 50 μ g of DEP for 20 days. Ten days prior to DEP instillation, animals were pretreated orally with 50, 150, or 250 mg/kg of anacardic acids or vehicle (100 μ L of cashew nut oil) for 30 days. The biomarkers of inflammatory and antioxidant responses in the alveolar parenchyma, bronchoalveolar lavage fluid (BALF), and pulmonary vessels were investigated. All doses of anacardic acids ameliorated antioxidant enzyme activities and decreased vascular adhesion molecule in vessels. Animals that received 50 mg/kg of anacardic acids showed decreased levels of neutrophils and tumor necrosis factor in the lungs and BALF, respectively. In summary, we demonstrated that AAs supplementation has a potential protective role on oxidative and inflammatory mechanisms in the lungs.
腰果酚来自巴西天然物质腰果壳液，具有抗菌和抗氧化活性，可调节免疫反应和血管生成。由于炎症性肺病与环境污染物暴露有关，并且没有证据表明饮食补充腰果酚对体内肺炎症有影响，我们研究了补充腰果酚在柴油机排气颗粒（DEP）诱导的肺炎症模型中的作用。BALB/c 小鼠接受了 50 μ g 的 DEP 的鼻腔内滴注 20 天。在 DEP 滴注前 10 天，动物用 50、150 或 250mg/kg 的腰果酚或载体（100 μ L 腰果油）进行了 30 天的口服预处理。研究了肺泡实质、支气管肺泡灌洗液（BALF）和肺血管中的炎症和抗氧化反应的生物标志物。所有剂量的腰果酚均可改善抗氧化酶活性并降低血管中的血管粘附分子。接受 50mg/kg 腰果酚的动物分别显示肺部和 BALF 中的中性粒细胞和肿瘤坏死因子水平降低。总之，我们证明了 AAs 补充具有对肺部氧化和炎症机制的潜在保护作用。
