软体动物神经元爆发式起搏电位活动的研究。II. 二价阳离子的调节作用。

Studies on bursting pacemaker potential activity in molluscan neurons. II. Regulations by divalanet cations.

作者信息

Barker J L, Gainer H

出版信息

Brain Res. 1975 Feb 14;84(3):479-500. doi: 10.1016/0006-8993(75)90767-2.

Abstract

Identified cells in Aplysia (R15, R2, LPG) and cell 11 in Otala have been used to investigate the effects of divalent cations, temperature, pH and ouabain on neuronal activity. Divalent cations act primarily to regulate the appearance of bursting pacemaker potential (BPP) activity in these cells. These ions are necessary for B generation and will inhibit its appearance at high concentrations (Ca greater than Mg greater that greater than Sr). In addition, Ca is involved in the seasonal modulation of BPP activity in a neurosecretory cell in Otala. Monovalent cations play secondary roles as regulators of BPP activity by competing with divalent cations for the sites involved in the regulation of the (probable) monovalent conductances underlying BPPs. The effects of pH, temperature and ouabain on membrane properties and BPP activity are partly related to their interaction with divalent cations. The results described indicate important roles for divalent cations in the regulation of the expression of BPP activity and its underlying membrane properties both in different nerve cells and in the same cell during dormancy and activity of the snails.

摘要

已利用海兔中的特定细胞（R15、R2、LPG）和玉黍螺中的11号细胞来研究二价阳离子、温度、pH值和哇巴因对神经元活动的影响。二价阳离子主要作用是调节这些细胞中爆发性起搏电位（BPP）活动的出现。这些离子是产生BPP所必需的，并且在高浓度时（钙大于镁大于锶）会抑制其出现。此外，钙参与了玉黍螺神经分泌细胞中BPP活动的季节性调节。单价阳离子通过与二价阳离子竞争参与调节BPP潜在的（可能的）单价电导的位点，从而在调节BPP活动中起次要作用。pH值、温度和哇巴因对膜特性和BPP活动的影响部分与其与二价阳离子的相互作用有关。所述结果表明，二价阳离子在不同神经细胞以及蜗牛休眠和活动期间同一细胞中调节BPP活动的表达及其潜在膜特性方面发挥着重要作用。