Department of General Surgery, State Hospital, Adıyaman, Turkey.
Libyan J Med. 2013 Mar 26;8(1):20596. doi: 10.3402/ljm.v8i0.20596.
There is no valid and reliable diagnostic test for early diagnosis of acute mesenteric ischemia (AMI). The aim of this study was to measure the plasma levels of diamine oxidase (DAO) and citrulline in AMI to gain insight into its early diagnosis.
A total of 21 Wistar albino rats were divided into three groups, that is, control group, short-term ischemia group, and prolonged ischemia group. The superior mesenteric artery was occluded for 15 min in the short-term ischemia group and for 12 h in the prolonged ischemia group. Twelve hours later, the experiment was terminated and plasma DAO and citrulline levels were measured. Intestinal tissue was evaluated for the histopathological changes.
Compared to the control group, the short-term and prolonged ischemia groups showed significant increases in the plasma levels of DAO, whereas the plasma citrulline levels decreased significantly. Prolonged ischemia caused a larger increase in the plasma DAO levels and a larger decrease in the plasma citrulline levels compared to the short-term ischemia (p=0.011 and p=0.021, respectively). Intestinal damage was shown to develop more in the prolonged ischemia group (p=0.001).
In the early period of AMI, the plasma DAO levels increase while citrulline levels decrease, and the extent of these changes depends on the duration of ischemia.
目前尚无有效的、可靠的诊断试验用于急性肠系膜缺血（AMI）的早期诊断。本研究旨在测量 AMI 患者的血浆二胺氧化酶（DAO）和瓜氨酸水平，以期深入了解其早期诊断。
将 21 只 Wistar 白化大鼠随机分为三组，即对照组、短期缺血组和长期缺血组。在短期缺血组中，肠系膜上动脉闭塞 15 分钟，在长期缺血组中闭塞 12 小时。12 小时后，终止实验并测量血浆 DAO 和瓜氨酸水平。评估肠组织的组织病理学变化。
与对照组相比，短期和长期缺血组的血浆 DAO 水平显著升高，而血浆瓜氨酸水平显著降低。与短期缺血相比，长期缺血导致血浆 DAO 水平升高更大，血浆瓜氨酸水平降低更大（p=0.011 和 p=0.021）。长期缺血组肠损伤更严重（p=0.001）。
在 AMI 的早期阶段，血浆 DAO 水平升高，瓜氨酸水平降低，这些变化的程度取决于缺血持续时间。
