Zhang Nanbin, Li Xianghui, Xu Huijie, Li Fuchang, Liu Lei
Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding, Department of Animal Science and Technology, Shandong Agricultural University, Taian 271017, China.
Animals (Basel). 2025 Jun 29;15(13):1923. doi: 10.3390/ani15131923.
The present study was conducted to investigate the effects of dietary addition of tributyrin on growth performance, antioxidant and intestinal health in weaned rabbits. Weaned Hyla rabbits (35 d, = 1280) of similar body weight were randomly divided into four groups (eight replicates per group, 40 rabbits per replicate), fed diets with 0, 0.1%, 0.2% or 0.4% tributyrin addition. A 3-day pre-experimental period was followed by a 36-day trial period. Our results show that dietary addition of 0.2% tributyrin significantly increased the average daily gain (ADG) and final body weight (FBW) of rabbits, and decreased the diarrhea rate ( < 0.05). Dietary addition of 0.1% and 0.2% tributyrin significantly increased the average carcass weight and liver weight ( < 0.05). In the liver, dietary addition of tributyrin increased the content of glutathione peroxidase (GSH-PX) and total superoxide dismutase (T-SOD) compared with the control group ( < 0.05). Dietary addition of 0.1% and 0.2% tributyrin significantly increased the serum GSH-PX level ( < 0.05). Dietary addition of 0.2% tributyrin significantly increased the serum T-SOD level ( < 0.05). Dietary addition of 0.2% tributyrin significantly increased villus height/crypt depth (V/C) in the duodenum ( < 0.05). Dietary addition of 0.1-0.4% tributyrin significantly increased V/C in the jejunum ( < 0.05). Dietary addition of 0.1-0.2% tributyrin significantly increased the V/C in the ileum, but significantly decreased the content of serum diamine oxidase (DAO) and of D-lactic acid in serum ( < 0.05). In conclusion, dietary addition of tributyrin can improve the production performance and antioxidant ability of liver and serum, and improve the intestinal health by decreasing the diarrhea rate and the intestinal permeability and improving intestinal morphology in weaned rabbits. Based on the present results, the optimal level of tributyrin is 0.2% in the weaned rabbit diet.
本研究旨在探讨日粮中添加丁酸甘油酯对断奶仔兔生长性能、抗氧化能力及肠道健康的影响。将体重相近的断奶海拉兔（35日龄，n = 1280）随机分为四组（每组8个重复，每个重复40只兔），分别饲喂添加0、0.1%、0.2%或0.4%丁酸甘油酯的日粮。预试验期3天，随后是36天的试验期。结果表明，日粮中添加0.2%丁酸甘油酯显著提高了仔兔的平均日增重（ADG）和末体重（FBW），并降低了腹泻率（P < 0.05）。日粮中添加0.1%和0.2%丁酸甘油酯显著提高了平均胴体重和肝脏重量（P < 0.05）。在肝脏中，与对照组相比，日粮中添加丁酸甘油酯提高了谷胱甘肽过氧化物酶（GSH-PX）和总超氧化物歧化酶（T-SOD）的含量（P < 0.05）。日粮中添加0.1%和0.2%丁酸甘油酯显著提高了血清GSH-PX水平（P < 0.05）。日粮中添加0.2%丁酸甘油酯显著提高了血清T-SOD水平（P < 0.05）。日粮中添加0.2%丁酸甘油酯显著提高了十二指肠绒毛高度/隐窝深度（V/C）（P < 0.05）。日粮中添加0.1 - 0.4%丁酸甘油酯显著提高了空肠V/C（P < 0.05）。日粮中添加0.1 - 0.2%丁酸甘油酯显著提高了回肠V/C，但显著降低了血清二胺氧化酶（DAO）含量和血清D-乳酸含量（P < 0.05）。综上所述，日粮中添加丁酸甘油酯可提高断奶仔兔的生产性能和肝脏及血清的抗氧化能力，并通过降低腹泻率和肠道通透性以及改善肠道形态来改善肠道健康。基于目前的结果，断奶仔兔日粮中丁酸甘油酯的最佳添加水平为0.2%。
