Dybicz Monika, Gierczak Anna, Dąbrowska Julia, Rdzanek Łukasz, Michałowicz Bogdan
Department of General Biology and Parasitology, 5 Chałubińskiego Str., 02-004 Warsaw, Medical University of Warsaw, Poland.
Parasitol Int. 2013 Aug;62(4):364-7. doi: 10.1016/j.parint.2013.03.005. Epub 2013 Mar 25.
The identity of the causative agent of cystic echinococcosis (CE) in humans from central Poland receiving treatment between 2000 and 2010 was determined. A total of 47 samples obtained after hepatectomy were examined and protoscoleces were identified in wet preparations in 27 cases. Using DNA extracted from the samples, two mitochondrial regions (nad1 and cox1 genes) were amplified and the nad1 fragment was sequenced. This PCR analysis confirmed the presence of Echinococcus species in 30 cases and nad1 sequence alignments showed identity with the G7 (pig) strain, Echinococcus canadensis. These data demonstrate that the pig strain of this parasite is the most frequent causative agent of human cystic echinococcosis in central Poland.
确定了2000年至2010年间在波兰中部接受治疗的人类囊性棘球蚴病（CE）病原体的身份。对肝切除术后获得的47个样本进行了检查，在27例的湿制标本中鉴定出原头节。使用从样本中提取的DNA，扩增了两个线粒体区域（nad1和cox1基因），并对nad1片段进行了测序。该聚合酶链反应（PCR）分析证实30例中存在棘球绦虫属物种，nad1序列比对显示与G7（猪）株加拿大棘球绦虫相同。这些数据表明，这种寄生虫的猪株是波兰中部人类囊性棘球蚴病最常见的病原体。
