Hernández-Chea Roderico, Aragón-Méndez Américo Yoel, Hun Alejandro, Morales-Ramírez Paola, Silva Ilde, Villatoro Federico, Wassermann Marion
Dirección Departamental de Redes Integradas de Servicios de Salud de Guatemala, Área Sur, Ministerio de Salud Pública y Asistencia Social, Amatitlán, Guatemala.
Facultad de Medicina Veterinaria y Zootecnia, Universidad de San Carlos de Guatemala, Guatemala, Guatemala.
Parasitology. 2025 Feb;152(2):205-216. doi: 10.1017/S0031182025000150.
is the causative agent of cystic echinococcosis (CE), a globally distributed zoonotic infection. In Guatemala, no new data have been reported for the past 80 years on CE. To address this gap, a cross-sectional study at the municipal slaughterhouse of Quetzaltenango was conducted from March to August 2022 to determine the presence of sp. in backyard pigs. Moreover, the species and haplotypes, fertility status of hydatid cysts, association of fertility of the cysts to the sex of the pig and the size of cysts were investigated. For this purpose, 117 pigs were examined post-mortem, and cysts were extracted from their organs. Species identification was performed using nested polymerase chain reaction targeting the gene, and a haplotype network was constructed. Generalized linear models (GLMs) were applied to assess correlation between cysts fertility, sex of the pig and diameter of the cyst. The study revealed a high prevalence of 38·46% of CE, and a minimum prevalence of of 4·27%. Genetic characterization confirmed the presence of of the G7 haplogroup. Eight haplotypes unique to Guatemala were identified, along with one of global occurrence. Cysts from male pigs were 3·6 times more likely to be fertile than those from female pigs. A quadratic GLM determined that cysts with a diameter range of 2·09-4·20 cm had a higher probability of being fertile. The high prevalence of CE and the diversity of Guatemalan haplotypes confirm the endemicity of in this region.
是囊性棘球蚴病（CE）的病原体，这是一种全球分布的人畜共患感染病。在危地马拉，过去80年没有关于CE的新数据报告。为填补这一空白，于2022年3月至8月在克萨尔特南戈市屠宰场进行了一项横断面研究，以确定后院猪体内是否存在 种。此外，还研究了包虫囊肿的种类和单倍型、生育状况、囊肿生育能力与猪的性别以及囊肿大小的关联。为此，对117头猪进行了死后检查，并从其器官中提取囊肿。使用针对 基因的巢式聚合酶链反应进行物种鉴定，并构建了单倍型网络。应用广义线性模型（GLMs）评估囊肿生育能力、猪的性别和囊肿直径之间的相关性。研究显示CE的高流行率为38.46%，最低流行率为4.27%。基因特征证实存在G7单倍型群的 。鉴定出8种危地马拉特有的单倍型以及一种全球存在的单倍型。雄性猪的囊肿生育可能性是雌性猪的3.6倍。二次GLM确定直径范围为2.09 - 4.20厘米的囊肿生育可能性更高。CE的高流行率和危地马拉单倍型的多样性证实了该地区 病的地方性流行。
