Xia Xiaohua, Huo Weiran, Wan Ruyan, Zhang Linxia, Xia Xiaopei, Chang Zhongjie
College of Life Science, Henan Normal University, Xinxiang, Henan, People's Republic of China.
Int J Dev Biol. 2017;61(8-9):565-570. doi: 10.1387/ijdb.170055xx.
Sox3 is a single-exon gene located on the X chromosome in most vertebrates. It belongs to the SoxB1 subfamily, which is part of the larger Sox family. Previous studies have revealed that Sox3 is expressed in many fish species. However, how Sox3 influences the development of Misgurnus anguillicaudatus remains unknown. In this study, a Sox3 homologue, termed MaSox3, was cloned from the brain of M. anguillicaudatus using homology-based cloning and the rapid amplification of cDNA ends method. Sequence analysis reveals that MaSox3 encodes a hydrophilic protein, which contains a characteristic HMG-box DNA-binding domain of 79 amino acids, and shares high homology with Sox3 in other species. Additionally, quantitative real-time reverse transcription PCR and in situ hybridization showed that MaSox3 is consistently expressed during embryogenesis, with peak expression during the neurula stage and broad expression in the central nervous system. Moreover, tissue distribution analyses have revealed that MaSox3 is abundant in the adult brain, the particle cell layer, and the gonad. Additionally, its expression is observed in primary spermatocyte cells, primary oocytes and previtellogenic oocyte cells. Taken together, all of these results suggest that the expression of the MaSox3 gene is highly conserved during vertebrate evolution and involved in a wide range of developmental processes including embryogenesis, neurogenesis and gonad development.
Sox3是大多数脊椎动物中位于X染色体上的单外显子基因。它属于SoxB1亚家族，是更大的Sox家族的一部分。先前的研究表明，Sox3在许多鱼类物种中都有表达。然而，Sox3如何影响泥鳅的发育仍然未知。在本研究中，使用基于同源性克隆和cDNA末端快速扩增方法，从泥鳅脑中克隆了一个Sox3同源物，命名为MaSox3。序列分析表明，MaSox3编码一种亲水性蛋白，该蛋白包含一个由79个氨基酸组成的特征性HMG-box DNA结合结构域，并且与其他物种的Sox3具有高度同源性。此外，定量实时逆转录PCR和原位杂交表明，MaSox3在胚胎发育过程中持续表达，在神经胚阶段表达达到峰值，并且在中枢神经系统中广泛表达。此外，组织分布分析表明，MaSox3在成年脑、颗粒细胞层和性腺中含量丰富。此外，在初级精母细胞、初级卵母细胞和卵黄发生前的卵母细胞中也观察到其表达。综上所述，所有这些结果表明，MaSox3基因的表达在脊椎动物进化过程中高度保守，并参与了包括胚胎发生、神经发生和性腺发育在内的广泛发育过程。
