Matemity and Child Health Institute of Shaanxi Province, Shaanxi, Xi'an 710003, PR China.
Cell Biol Int. 2013 Jul;37(7):737-41. doi: 10.1002/cbin.10104. Epub 2013 May 13.
Cervical squamous carcinoma is a highly invasive tumour that has a great capacity to metastasise. Extracellular matrix metalloproteinase inducer (EMMPRIN or CD147), a member of the immunoglobulin superfamily, is a widely distributed cell surface glycoprotein. It is highly expressed on malignant tumour cell surfaces, including human cervical squamous carcinoma. It also plays a critical role in the invasive and metastatic activity of malignant cells by stimulating the expression of matrix metalloproteinases (MMPs). The anti-invasive effect of small interfering RNA (siRNA) against CD147 on human cervical squamous carcinoma cells and its possible pathways has been investigated. The downregulation of CD147 by transfection with siRNA resulted in MMP-9 expression and decreased activity in the cervical squamous carcinoma cell line SiHa. In vitro analysis showed that the invasive capacity of SiHa cells decreased. Thus CD147 inhibition and subsequent MMP-9 deletion may have anti-tumour effects by inhibiting the invasiveness of human cervical squamous carcinoma cells.
宫颈鳞状细胞癌是一种具有高度侵袭性和转移能力的肿瘤。细胞外基质金属蛋白酶诱导因子（EMMPRIN 或 CD147）是免疫球蛋白超家族的一员，是一种广泛分布的细胞表面糖蛋白。它在恶性肿瘤细胞表面高度表达，包括人宫颈鳞状细胞癌。它还通过刺激基质金属蛋白酶（MMPs）的表达，在恶性细胞的侵袭和转移活性中发挥关键作用。已经研究了针对 CD147 的小干扰 RNA（siRNA）对人宫颈鳞状细胞癌细胞的抗侵袭作用及其可能的途径。通过 siRNA 转染下调 CD147 导致宫颈鳞状癌细胞系 SiHa 中 MMP-9 的表达和活性降低。体外分析表明 SiHa 细胞的侵袭能力下降。因此，CD147 抑制和随后的 MMP-9 缺失可能通过抑制人宫颈鳞状癌细胞的侵袭性发挥抗肿瘤作用。
