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蜂毒肽通过下调CD147和MMP-9的表达来抑制MCF-7细胞的侵袭。

Melittin inhibits the invasion of MCF-7 cells by downregulating CD147 and MMP-9 expression.

作者信息

Wang Jianjun, Li Fengyu, Tan Jiang, Peng Xuewei, Sun Lili, Wang Ping, Jia Shengnan, Yu Qingmiao, Huo Hongliang, Zhao Hongyan

机构信息

Laboratory of Molecular and Cellular Physiology, School of Life Science, Northeast Normal University, Changchun, Jilin 130024, P.R. China.

出版信息

Oncol Lett. 2017 Feb;13(2):599-604. doi: 10.3892/ol.2016.5516. Epub 2016 Dec 20.

DOI:10.3892/ol.2016.5516
PMID:28356935
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5351397/
Abstract

Tumor invasion and metastasis are the critical steps in determining the aggressive phenotype of human cancers. Melittin, a major component of bee venom, has been reported to induce apoptosis in several cancer cells. However, the mechanisms of melittin involvement in cancer invasion and metastasis remain unclear. Our previous study indicated that melittin inhibits cyclophilin A (CypA), a ubiquitously distributed peptidylprolyl isomerase, in macrophage cells. In the present study, the Transwell assay results showed that melittin may downregulate the invasion level of MCF-7 cells in a dose-dependent manner. Additionally, it was also found, using flow cytometry and reverse transcription-polymerase chain reaction, that melittin decreased the expression of cluster of differentiation (CD)147 and matrix metallopeptidase-9 (MMP-9), whereas CypA upregulated the expression of CD147 and MMP-9. Overall, the present study indicated that melittin decreased the invasion level of MCF-7 cells by downregulating CD147 and MMP-9 by inhibiting CypA expression. The results of the present study provide an evidence for melittin in anticancer therapy and mechanisms.

摘要

肿瘤侵袭和转移是决定人类癌症侵袭性表型的关键步骤。蜂毒的主要成分蜂毒素已被报道可诱导多种癌细胞凋亡。然而，蜂毒素参与癌症侵袭和转移的机制仍不清楚。我们之前的研究表明，蜂毒素在巨噬细胞中抑制亲环素A（CypA），一种广泛分布的肽基脯氨酰异构酶。在本研究中，Transwell实验结果表明，蜂毒素可能以剂量依赖性方式下调MCF-7细胞的侵袭水平。此外，通过流式细胞术和逆转录-聚合酶链反应还发现，蜂毒素降低了分化簇（CD）147和基质金属蛋白酶-9（MMP-9）的表达，而CypA上调了CD147和MMP-9的表达。总体而言，本研究表明，蜂毒素通过抑制CypA表达下调CD147和MMP-9，从而降低MCF-7细胞的侵袭水平。本研究结果为蜂毒素在抗癌治疗及机制方面提供了证据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970e/5351397/4c3d4d0cf49c/ol-13-02-0599-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970e/5351397/b255cce6fb42/ol-13-02-0599-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970e/5351397/d9b0c43abd3c/ol-13-02-0599-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970e/5351397/4c3d4d0cf49c/ol-13-02-0599-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970e/5351397/b255cce6fb42/ol-13-02-0599-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970e/5351397/d9b0c43abd3c/ol-13-02-0599-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/970e/5351397/4c3d4d0cf49c/ol-13-02-0599-g02.jpg

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