丙型肝炎病毒核心蛋白对 miR-122 表达的影响。
The effects of hepatitis C virus core protein on the expression of miR-122 in vitro.
机构信息
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Institute of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
出版信息
Virol J. 2013 Mar 27;10:98. doi: 10.1186/1743-422X-10-98.
BACKGROUND
Hepatitis C virus (HCV) is one of the major pathogens of liver diseases. Some studies have previously reported that miR-122 can stimulate replication or translation of HCV. However, the effects of HCV infection on miR-122 expression are not clear. The aim of this study was to investigate the effects of HCV core protein on the expression of miR-122 in a cell culture model.
RESULTS
The miR-122 levels in Huh7.5.1 cells infected with HCV for different days or different HCV abundance were measured by real-time PCR. Significant decrease of miR-122 expression was found at late stage of infection and in the high-abundance group. Huh7.5.1 cells transfected with plasmid pEGFP-core or pEGFP were used to detect the effects of HCV core protein on miR-122 expression, the results showed that core protein could down-regulate the miR-122 expression level in a time- and dose- dependent manner, and reduced the susceptibility of Huh7.5.1 cell to HCV.
CONCLUSIONS
Down-regulating miR-122 expression by HCV core protein may give a new insight into the interaction between HCV and miR-122 and chronic HCV infection.
背景
丙型肝炎病毒 (HCV) 是肝脏疾病的主要病原体之一。一些研究先前报道 miR-122 可以刺激 HCV 的复制或翻译。然而，HCV 感染对 miR-122 表达的影响尚不清楚。本研究旨在探讨 HCV 核心蛋白在细胞培养模型中对 miR-122 表达的影响。
结果
通过实时 PCR 测定感染 HCV 不同天数或 HCV 丰度不同的 Huh7.5.1 细胞中的 miR-122 水平。在感染后期和高丰度组中发现 miR-122 表达明显下降。转染质粒 pEGFP-core 或 pEGFP 的 Huh7.5.1 细胞用于检测 HCV 核心蛋白对 miR-122 表达的影响，结果表明核心蛋白可以时间和剂量依赖的方式下调 miR-122 的表达水平，并降低 Huh7.5.1 细胞对 HCV 的易感性。
结论
HCV 核心蛋白下调 miR-122 表达可能为 HCV 和 miR-122 之间的相互作用以及慢性 HCV 感染提供新的见解。
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