使用经二氧化碳和氧张力计测量的磷酸盐 - 碳酸氢盐 - 氯化物 - 甘油 - 水混合物对pH、pCO2和pO2电极系统进行校准和控制。

Use of carbon dioxide- and oxygen-tonometered phosphate-bicarbonate-chloride-glycerol-water mixtures for calibration and control of pH, pCO2, and pO2 electrode systems.

作者信息

Veefkind A H, Van den Camp R A, Maas A H

出版信息

Clin Chem. 1975 May;21(6):685-93.

Abstract

Calibration of pH, PCO2, and PO2 electrode systems of modern blood-gas analyzers, designed with one sample cuvet for measurement, is mostly performed separately with buffer solutions of known pH, PCO2, and PO2 for doing such calibrations simultaneously, containing phosphate, bicarbonate, and chloride in glycerol-water mixtures as solvent. A method is suggested for computing the relation between pH and log PCO2 of these solutions in equilibrium with carbon dioxide gas. It is demonstrated that a solution of phosphate (Na2HPO4, KH2PO4, each 25 mmol/liter), bicarbonate (NaHCO3, 30 mmol/liter), and chloride (Nacl, 30 mmol/liter) in glycerol-water mixture (3/7 by vol) and equilibrated with CO2 in air (4 vol/100 vol) and CO2 in nitrogen (8 vol/100 vol), respectively, makes possible acurate and simultaneous calibration of the pH, PCO2, PO2 electrodes of a Corning Model 165 blood-gas analyzer. Similar solutions may also be used for quality-control of blood-gas measurement.

摘要

现代血气分析仪的pH、PCO₂和PO₂电极系统的校准，这些仪器设计为用一个样品比色皿进行测量，大多是分别使用已知pH、PCO₂和PO₂的缓冲溶液进行校准，而不是同时进行校准，这些缓冲溶液在甘油 - 水混合物作为溶剂中含有磷酸盐、碳酸氢盐和氯化物。本文提出了一种计算这些与二氧化碳气体处于平衡状态的溶液的pH与log PCO₂之间关系的方法。结果表明，由磷酸盐（Na₂HPO₄、KH₂PO₄，各25 mmol/升）、碳酸氢盐（NaHCO₃，30 mmol/升）和氯化物（NaCl，30 mmol/升）组成的溶液，在甘油 - 水混合物（体积比3/7）中，分别与空气中的CO₂（4体积/100体积）和氮气中的CO₂（8体积/100体积）平衡后，能够对康宁165型血气分析仪的pH、PCO₂、PO₂电极进行准确且同时的校准。类似的溶液也可用于血气测量的质量控制。