Kawahara Hidehisa, Hirai Akihito, Minabe Toshi, Obata Hitoshi
Department of Life Science and Biotechnology, Kansai University, Osaka, Japan.
Biocontrol Sci. 2013;18(1):21-8. doi: 10.4265/bio.18.21.
We found that a novel biosurfactant from the cultured broth of red yeast, Rhodotorula mucilaginosa KUGPP-1, originating in the Antarctic, has dispersive power against astaxanthin. The novel biosurfactant was purified from extracts to the ultrafiltration state by acetone precipitation and chromatography on a DEAE-Toyopearl 650 M, and gel filtration on a Sephacryl S-400 HR. The molecular mass of the novel biosurfactant was estimated to be about 730,000 by gel filtration chromatography. The novel biosurfactant was comprised of sugar and protein in an approximate molar ratio of 9 : 1. The sugars were comprised of mannose, galactose and glucose. The particle size of the astaxanthin (0.13 µ g/ml) micelle was about 410 nm. Astaxanthin was stable to oxidation in the novel biosurfactant micelles. To our knowledge, this is the first report on a glycoprotein type of biosurfactant with astaxanthin-stabilizing ability.
我们发现，从源自南极的粘红酵母Rhodotorula mucilaginosa KUGPP - 1培养肉汤中提取的一种新型生物表面活性剂对虾青素具有分散能力。通过丙酮沉淀、DEAE - Toyopearl 650 M柱色谱以及Sephacryl S - 400 HR凝胶过滤，将该新型生物表面活性剂从提取物中纯化至超滤状态。通过凝胶过滤色谱法估计该新型生物表面活性剂的分子量约为730,000。该新型生物表面活性剂由糖和蛋白质组成，摩尔比约为9 : 1。糖类由甘露糖、半乳糖和葡萄糖组成。虾青素（0.13 µg/ml）胶束的粒径约为410 nm。虾青素在新型生物表面活性剂胶束中对氧化稳定。据我们所知，这是关于具有虾青素稳定能力的糖蛋白型生物表面活性剂的首次报道。
