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从盐田土壤中分离的一株海洋细菌 YL-1 胞外多糖的发酵、性质及免疫活性研究

Production, Characterization and Immunomodulatory Activity of an Extracellular Polysaccharide from YL-1 Isolated from Sea Salt Field.

机构信息

College of Medicine, Hangzhou Normal University, Hangzhou 311121, China.

Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.

出版信息

Mar Drugs. 2020 Nov 26;18(12):595. doi: 10.3390/md18120595.

DOI:10.3390/md18120595
PMID:33256151
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7760879/
Abstract

A novel exopolysaccharide from marine-derived red yeast strain YL-1 was produced and characterized. The highest yield of polysaccharide reached 15.1 g/L after medium and culture parameter optimization. This exopolysaccharide, composed of four neural monosaccharides including glucose, mannose, galactose and fucose, had an average molecular weight of 1200 KDa. It had good immunomodulatory activity on RAW256.7 cell lines. ELISA (enzyme linked immunosorbent assay) and Q-PCR (quantitative real-time PCR) results showed that the cell was stimulated to express more IL-6, IL-18, IL-1β and TNFα cytokines than the control group. This is the first report of an exopolysaccharide with immunomodulatory activity from marine-derived .

摘要

一株海洋来源红酵母 YL-1 产生并鉴定了一种新型胞外多糖。经过培养基和培养参数优化，多糖产量最高可达 15.1 g/L。该胞外多糖由葡萄糖、甘露糖、半乳糖和岩藻糖四种单糖组成，平均分子量为 1200 kDa。它对 RAW256.7 细胞系具有良好的免疫调节活性。ELISA（酶联免疫吸附测定）和 Q-PCR（实时定量 PCR）结果表明，与对照组相比，该细胞被刺激表达更多的细胞因子 IL-6、IL-18、IL-1β 和 TNFα。这是首次报道海洋来源的具有免疫调节活性的胞外多糖。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/87c113e1a680/marinedrugs-18-00595-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/6de91b098c9b/marinedrugs-18-00595-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/266e0511400f/marinedrugs-18-00595-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/0c62047bcc73/marinedrugs-18-00595-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/63a5089c8092/marinedrugs-18-00595-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/3db0593f63d4/marinedrugs-18-00595-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/48883b1a2649/marinedrugs-18-00595-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/780e5e4e450e/marinedrugs-18-00595-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/ea0e6c153707/marinedrugs-18-00595-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/aa47fc7f7d1a/marinedrugs-18-00595-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/87c113e1a680/marinedrugs-18-00595-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/6de91b098c9b/marinedrugs-18-00595-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/266e0511400f/marinedrugs-18-00595-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/0c62047bcc73/marinedrugs-18-00595-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/63a5089c8092/marinedrugs-18-00595-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/3db0593f63d4/marinedrugs-18-00595-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/48883b1a2649/marinedrugs-18-00595-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/780e5e4e450e/marinedrugs-18-00595-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/ea0e6c153707/marinedrugs-18-00595-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/aa47fc7f7d1a/marinedrugs-18-00595-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3771/7760879/87c113e1a680/marinedrugs-18-00595-g010.jpg

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