Department of Clinical Chemistry, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands.
Rev Endocr Metab Disord. 2013 Jun;14(2):159-84. doi: 10.1007/s11154-013-9241-0.
The prevalence of vitamin D deficiency in the general population has become a major public health problem. Vitamin D deficiency might have significant consequences not only to bone health but possibly to autoimmune-, infectious and cardiovascular disease. This has resulted in increased clinical testing for 25-hydroxyvitamin D (25(OH)D) in serum, as circulating 25(OH)D is regarded as the best indicator of adequate exposure to sunlight and dietary intake of vitamin D. There are reportedly over 50 vitamin D metabolites of which 25(OH)D and 1,25(OH)2D are well known to provide clinical information. More recently, there is increasing interest in measuring the C3-epimer of 25(OH)D, which has shown to contribute significantly to the 25(OH)D concentration, particularly in infant populations, and in 24,25(OH)2D, a major catabolite of 25(OH)D metabolism. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is an analytical tool that allows the specific determination of all relevant vitamin D metabolites, with the potential of performing multiple analyte analysis in a single experimental setting, creating a vitamin D profile. This article reviews recent advances in the quantification of vitamin D metabolites using LC-MS/MS.
维生素 D 缺乏症在普通人群中的流行已成为一个主要的公共卫生问题。维生素 D 缺乏不仅对骨骼健康有重大影响，而且可能对自身免疫性疾病、感染和心血管疾病有影响。这导致人们对血清 25-羟维生素 D（25(OH)D）的临床检测增加，因为循环中的 25(OH)D 被认为是暴露于阳光和维生素 D 饮食摄入充足的最佳指标。据报道，有超过 50 种维生素 D 代谢物，其中 25(OH)D 和 1,25(OH)2D 是众所周知的提供临床信息的代谢物。最近，人们越来越关注测量 25(OH)D 的 C3-差向异构体，它对 25(OH)D 浓度的贡献很大，特别是在婴儿群体中，以及 24,25(OH)2D，它是 25(OH)D 代谢的主要分解产物。液相色谱-串联质谱（LC-MS/MS）是一种分析工具，可特异性测定所有相关的维生素 D 代谢物，具有在单个实验条件下进行多种分析物分析的潜力，从而创建维生素 D 图谱。本文综述了使用 LC-MS/MS 定量测定维生素 D 代谢物的最新进展。
