Noorafshan Ali, Karbalay-Doust Saied, Poorshahid Mohammad
Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Acta Clin Croat. 2012 Dec;51(4):555-62.
Hydrostatic pressure, which is the result of urinary tract blockage, initiates renal injuries. The injuries are characterized by tubular dilatation and/or atrophy, tubular cell death, inflammatory process and progressive interstitial fibrosis with loss of renal parenchyma. The aim of this study was to evaluate the ameliorative effects of sulforaphane and quercetin, the two natural compounds that can be found in vegetables, in unilateral ureteral obstruction (UUO). Three groups of rats underwent surgery to induce UUO. They received distilled water, sulforaphane (500 microg/animal/ day) and quercetin (50 mg/kg/day). Stereological methods were applied in order to obtain accurate, quantitative and comparable data. Less than approximately 4% of renal structures on average remained intact in UUO rats. After the treatment of UUO rats with quercetin, approximately 69%, 32%, 65%, 35% and 41% of the volume of the glomeruli, proximal and distal convoluted tubules (PCT and DCT), Henle's loop and collecting ducts remained intact, respectively (p < 0.01). After the treatment of UUO rats with sulforaphane, approximately 24%, 45%, and 26% of the volume of the PCT, DCT and Henle's loop remained intact, respectively (p < 0.01). After the treatment of UUO rats with quercetin, approximately 71%, 81%, 51%, and 57% of the length of the PCT, DCT, Henle's loop and collecting ducts remained intact, respectively (p < 0.01). After the treatment of UUO rats with sulforaphane, approximately 42% and 41% of the length of the PCT and DCT remained intact, respectively (p < 0.01). Changes in the length of Henle's loop and collecting ducts were not significant. In conclusion, quercetin and sulforaphane were found to be effective in preventing some structural renal damage in the direct obstruction model. Quercetin had a more ameliorative role on renal structures.
静水压是尿路梗阻的结果，可引发肾损伤。这些损伤的特征为肾小管扩张和/或萎缩、肾小管细胞死亡、炎症过程以及肾实质丧失导致的进行性间质纤维化。本研究的目的是评估萝卜硫素和槲皮素这两种可在蔬菜中找到的天然化合物对单侧输尿管梗阻（UUO）的改善作用。三组大鼠接受手术以诱导UUO。它们分别接受蒸馏水、萝卜硫素（500微克/动物/天）和槲皮素（50毫克/千克/天）。应用体视学方法以获得准确、定量且可比的数据。在UUO大鼠中，平均不到约4%的肾结构保持完整。用槲皮素治疗UUO大鼠后，肾小球、近端和远端曲管（PCT和DCT）、髓袢和集合管的体积分别约有69%、32%、65%、35%和41%保持完整（p<0.01）。用萝卜硫素治疗UUO大鼠后，PCT、DCT和髓袢的体积分别约有24%、45%和26%保持完整（p<0.01）。用槲皮素治疗UUO大鼠后，PCT、DCT、髓袢和集合管的长度分别约有71%、81%、51%和57%保持完整（p<0.01）。用萝卜硫素治疗UUO大鼠后，PCT和DCT的长度分别约有42%和41%保持完整（p<0.01）。髓袢和集合管长度的变化不显著。总之，发现槲皮素和萝卜硫素在直接梗阻模型中对预防一些肾脏结构损伤有效。槲皮素对肾脏结构具有更强的改善作用。
