Karbalay-Doust Saied, Noorafshan Ali, Pourshahid Seyed-Mohammad
Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Korean J Urol. 2012 May;53(5):360-7. doi: 10.4111/kju.2012.53.5.360. Epub 2012 May 18.
Blockage of the urinary tract induces changes in renal structure including tubular dilatation or atrophy, tubular cell death, inflammatory processes, and progressive interstitial fibrosis with the loss of renal parenchyma. The present study was conducted to survey the protective effects of Taxol and taurine on the renal structure after unilateral ureteral obstruction (UUO).
UUO was induced in three groups of rats (n=6) who then received distilled water, Taxol (0.3 mg/kg/d), or taurine (7.5 mg/kg/d). Stereological methods were used to gather quantitative as well as comparative data.
Less than -8% of the volume of the glomeruli, proximal convoluted tubules (PCT), distal convoluted tubules (DCT), Henle's loop, and collecting ducts were preserved after UUO. After treatment of the UUO rats with Taxol, between -32% and 88% of the parameters mentioned above remained intact, and after treatment of the UUO rats with taurine, between -16% and 46% of the parameters remained intact (p<0.01). Compared with the untreated UUO animals, the volume of necrotic and fibrotic tissues decreased -53% and -63% in the UUO rats treated with Taxol and taurine, respectively (p<0.01). Less than -3% of the lengths of the renal tubules (PCT, DCT, Henle's loop, and collecting) were preserved in the UUO rats. After treatment with Taxol and taurine, -61% to 70% and -43% to 53% of the length of the renal tubules were preserved, respectively (p<0.01).
Taurine and Taxol are effective in preventing some structural renal damage in a direct ureteral obstruction model. Taxol was more effective in renal protection.
尿路梗阻会引起肾脏结构改变，包括肾小管扩张或萎缩、肾小管细胞死亡、炎症反应以及肾实质丧失导致的进行性间质纤维化。本研究旨在探讨紫杉醇和牛磺酸对单侧输尿管梗阻（UUO）后肾脏结构的保护作用。
将三组大鼠（n = 6）诱导建立UUO模型，随后分别给予蒸馏水、紫杉醇（0.3 mg/kg/d）或牛磺酸（7.5 mg/kg/d）。采用体视学方法收集定量及对比数据。
UUO后，肾小球、近端小管（PCT）、远端小管（DCT）、髓袢和集合管的体积保留率低于-8%。用紫杉醇治疗UUO大鼠后，上述参数的保留率在-32%至88%之间；用牛磺酸治疗UUO大鼠后，参数保留率在-16%至46%之间（p<0.01）。与未治疗的UUO动物相比，用紫杉醇和牛磺酸治疗的UUO大鼠坏死和纤维化组织的体积分别减少了-53%和-63%（p<0.01）。UUO大鼠肾小管（PCT、DCT、髓袢和集合管）长度的保留率低于-3%。用紫杉醇和牛磺酸治疗后，肾小管长度的保留率分别为-61%至70%和-43%至53%（p<0.01）。
在直接输尿管梗阻模型中，牛磺酸和紫杉醇可有效预防部分肾脏结构损伤。紫杉醇的肾脏保护作用更显著。
