State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 155 Changbai Road, Changping District, Beijing, 102206, China.
Arch Virol. 2013 Sep;158(9):1865-76. doi: 10.1007/s00705-013-1676-9. Epub 2013 Mar 30.
The bacteriophage VP3 is used in a phage-biotyping scheme as one of the typing phages of Vibrio cholerae O1 biotype El Tor strains. Here, we have sequenced and analyzed its genome. The genome consists of 39,481 bp with an overall G + C content of 42.6 %. Fifty-two open reading frames (ORFs) were predicted. Within the genome, 17 highly conserved phage promoters and 6 rho-independent terminators were predicted. When assessed with Rluc as a reporter gene, 12 of 16 cloned VP3 promoters showed activity in the host strain V. cholerae biotype El Tor. Based on the temporal expression pattern detected using reverse transcription PCR (RT-PCR), VP3 ORFs can be classed into four groups, arranged according to their order in the VP3 genome. Terminators T1 and T6 are presumed to work efficiently. Sequencing of the typing phage VP3 of V. cholerae reveals its evolutionary subdivisions from the members of T7-like phages of Escherichia coli. Knowledge of VP3 expands the known host range of T7-like phages and will promote understanding the different infection mechanisms used by members of this genus.
噬菌体 VP3 被用于噬菌体生物分型方案中，作为霍乱弧菌 O1 生物型 El Tor 株的分型噬菌体之一。在这里，我们对其基因组进行了测序和分析。基因组由 39481bp 组成，总 G+C 含量为 42.6%。预测了 52 个开放阅读框（ORF）。在基因组内，预测了 17 个高度保守的噬菌体启动子和 6 个 rho 非依赖性终止子。当用 Rluc 作为报告基因进行评估时，在宿主菌株霍乱弧菌生物型 El Tor 中，16 个克隆的 VP3 启动子中的 12 个显示出活性。根据使用反转录 PCR（RT-PCR）检测到的时间表达模式，VP3 ORFs 可以根据它们在 VP3 基因组中的顺序分为四组。假定终止子 T1 和 T6 能有效工作。霍乱弧菌的分型噬菌体 VP3 的测序揭示了它与大肠杆菌 T7 样噬菌体成员的进化分支。对 VP3 的了解扩展了 T7 样噬菌体的已知宿主范围，并将促进理解该属成员使用的不同感染机制。
