The outer-membrane protein TolC of serves as a second cell-surface receptor for the VP3 phage.

Receptor recognition is a key step in the initiation of phage infection. Previously, we found that VP3, the T7 family phage of the serogroup O1 biotype El Tor, can adsorb the core oligosaccharide (OS) of lipopolysaccharides of However, some wildtype strains of possessing the intact OS gene cluster still have VP3 binding but are resistant to VP3 infection. Moreover, an OS gene-deletion mutant still exhibits weak VP3 binding, suggesting multiple factors are possibly involved in VP3 binding to Here, we report that the outer-membrane protein TolC of is involved in the host adsorption of VP3. We observed that TolC directly interacts with the VP3 tail fiber protein gp44 and its C-terminal domains, and we also found that three amino acid residues in the outside loops of TolC, at positions 78, 290, and 291, are critical for binding to gp44. Among the VP3-resistant wildtype strains, frequent amino acid residue mutations were observed in the loops around the sites 78, 290, and 291, which were predicted to be exposed to the cell surface. These findings reveal a co-receptor-binding mechanism for VP3 infection of and that both outer-membrane TolC and OS are necessary for successful VP3 infection of We conclude that mutations on the outside loops of the receptor may confer strains with VP3 phage resistance, enabling these strains to survive in environments containing VP3 or related phages.