Faculty of Resource and Environmental Sciences, Sichuan Agricultural University, Yaan 625000, PR China.
Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang Production and Construction Corps, Tarim University, Alar, Xinjiang 843300, PR China.
Int J Syst Evol Microbiol. 2010 Dec;60(Pt 12):2991-2995. doi: 10.1099/ijs.0.020214-0. Epub 2010 Jan 29.
A novel bacterium, designated TRM 415(T), belonging to the genus Brevibacterium, was isolated from a sediment sample from a salt lake in Xinjiang province, China. Comparative 16S rRNA gene sequence analysis indicated that strain TRM 415(T) was phylogenetically most closely related to Brevibacterium album YIM 90718(T) (98.4 % sequence similarity) and had low similarity (<95.5 %) to other species of the genus Brevibacterium; however, DNA-DNA hybridization studies between strain TRM 415(T) and B. album YIM 90718(T) showed only 41.3 % relatedness. Strain TRM 415(T) possessed meso-diaminopimelic acid as the diagnostic cell-wall diamino acid, MK-8(H(2)) as the major menaquinone and polar lipids including phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids were anteiso-C(17 : 0) and anteiso-C(15 : 0). The genomic DNA G+C content was 69 mol%. Based on the evidence from this polyphasic study, strain TRM 415(T) represents a novel species of the genus Brevibacterium, for which the name Brevibacterium salitolerans sp. nov. is proposed. The type strain is TRM 415(T) (=JCM 15900(T) =CCTCC AB 208328(T) =KCTC 19616(T)).
一株新的细菌，命名为 TRM 415(T)，属于短杆菌属，是从中国新疆盐湖的沉积物样本中分离出来的。比较 16S rRNA 基因序列分析表明，菌株 TRM 415(T)与 Brevibacterium album YIM 90718(T)（98.4%序列相似度）在系统发育上最为密切相关，与短杆菌属的其他种的相似性较低（<95.5%）；然而，TRM 415(T)与 B. album YIM 90718(T)之间的 DNA-DNA 杂交研究表明，它们之间只有 41.3%的相关性。菌株 TRM 415(T)具有 meso-二氨基庚二酸作为细胞壁二氨基庚二酸，MK-8(H(2))作为主要的menaquinone 和极性脂类，包括磷脂酰甘油和双磷脂酰甘油。主要脂肪酸为 anteiso-C(17 : 0)和 anteiso-C(15 : 0)。基因组 DNA G+C 含量为 69 mol%。基于多相研究的证据，菌株 TRM 415(T)代表了短杆菌属的一个新种，建议命名为 Brevibacterium salitolerans sp. nov.。模式菌株为 TRM 415(T)=JCM 15900(T)=CCTCC AB 208328(T)=KCTC 19616(T)。
