School of Marine Science and Biotechnology, Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi Minzu University, Nanning, 530008, People's Republic of China.
School of Chemistry and Chemical Engineering, Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, Guangxi Minzu University, Nanning, 530008, People's Republic of China.
Antonie Van Leeuwenhoek. 2024 Oct 14;118(1):19. doi: 10.1007/s10482-024-02031-2.
A Gram stain-positive, non-spore-forming, non-motile, short-rod actinomyces strain GXQ1321 was isolated from maritime surface sediments in Beihai (21° 41' 21.65″ N, 109° 05' 76.56″ E), Guangxi Zhuang Autonomous Region, and a number of categorization studies were performed. Following a period of 72 h of incubation at a temperature of 30 °C within a modified actinomycete culture medium, the colony was light-yellow, circular, smooth, central bulge, convex, opaque, with a 1.2-2.3 mm diameter. Strain GXQ1321 had the ability to degrade cellulose. Chemotaxonomic studies revealed that the major methylnaphthoquinones in strain GXQ1321 was MK-8(H). The most prevalent cellular fatty acids were anteiso-C, anteiso-C, anteiso-C, and iso-C. The whole-cell sugars of the strain GXQ1321 were identified rhamnose, xylose and glucose. Meso-diaminopimelic acid was found in the peptidoglycan hydrolysate, and the polar lipids were identified as diphosphatidylglycerol, three phosphoglycolipid, phosphatidylglycerol and two unknown glycolipid. This strain had 69.6% DNA G+C content. Strain GXQ1321 is classified as Brevibacterium based on its 16S rRNA gene sequence. It is closely related to Brevibacterium samyangense SST-8 (96.8%) and Brevibacterium rongguiense 5221 (96.3%). The average nucleotide identity (ANI) values of GXQ1321 and the above two type strains were 73.9-77.1%, and the digital DNA-DNA hybridisation (dDDH) values were 15.3-21.1%. Based on the phylogenetic, chemotaxonomic and physiological data, strain GXQ1321 was considered to be a novel species of the genus Brevibacterium, named Brevibacterium litoralis sp. nov, with the type strain GXQ1321 (= MCCC 1K08964 = KCTC 59167).
一株革兰氏阳性、非孢子形成、非运动、短杆放线菌菌株 GXQ1321 从广西壮族自治区北海市（北纬 21°41'21.65"，东经 109°05'76.56"）的海洋表层沉积物中分离出来，并进行了多项分类研究。在改良的放线菌培养基中于 30°C 培养 72 小时后，该菌落呈浅黄色，圆形，光滑，中央凸起，凸面，不透明，直径为 1.2-2.3mm。菌株 GXQ1321 具有降解纤维素的能力。化学生态研究表明，菌株 GXQ1321 中的主要甲基萘醌为 MK-8(H)。最常见的细胞脂肪酸为 anteiso-C、anteiso-C、anteiso-C 和 iso-C。菌株 GXQ1321 的全细胞糖鉴定为鼠李糖、木糖和葡萄糖。在肽聚糖水解物中发现了 meso-二氨基庚二酸，极性脂类鉴定为双磷脂酰甘油、三种磷酸甘油酯、磷脂酰甘油和两种未知糖脂。该菌株的 DNA G+C 含量为 69.6%。根据 16S rRNA 基因序列，菌株 GXQ1321 被归类为短杆菌属。它与 Brevibacterium samyangense SST-8（96.8%）和 Brevibacterium rongguiense 5221（96.3%）最为密切相关。GXQ1321 与上述两株模式菌株的平均核苷酸同一性（ANI）值为 73.9-77.1%，数字 DNA-DNA 杂交（dDDH）值为 15.3-21.1%。基于系统发育、化学分类和生理数据，菌株 GXQ1321 被认为是短杆菌属的一个新种，命名为 Brevibacterium litoralis sp. nov，其模式菌株为 GXQ1321（= MCCC 1K08964 = KCTC 59167）。
