Basic Science Research Institute, Kyonggi University, San 94-6, Iui-dong, Yeongtong-gu, Suwon-si, 443-760, Republic of Korea.
Department of Life Science, School of Natural Science, Kyonggi University, San 94-6, Iui-dong, Yeongtong-gu, Suwon-si, 443-760, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Mar;63(Pt 3):1111-1118. doi: 10.1099/ijs.0.039305-0. Epub 2012 Jun 22.
A Gram-stain-positive, non-motile, chemo-organotrophic, mesophilic, aerobic bacterium, designated A1(T), was isolated from sludge of a wastewater treatment plant. Strain A1(T) showed good ability to degrade ammonia and grew well on media amended with methanol and ammonia. Strain A1(T) grew with 0-11 % (w/v) NaCl, at 20-42 °C, but not <15 or >45 °C and at pH 6-10 (optimum pH 8.0-9.0). The isolate was catalase-positive and oxidase-negative. The DNA G+C content was 70.7 mol%. A comparative analysis of 16S rRNA gene sequences revealed that strain A1(T) formed a distinct phyletic lineage in the genus Brevibacterium and showed high sequence similarity with Brevibacterium casei NCDO 2048(T) (96.9 %), Brevibacterium celere KMM 3637(T) (96.9 %) and Brevibacterium sanguinis CF63(T) (96.4 %). DNA-DNA hybridization revealed <43 % DNA-DNA relatedness between the isolate and its closest phylogenetic relatives. The affiliation of strain A1(T) with the genus Brevibacterium was supported by the chemotaxonomic data: predominant quinone menaquinone MK-7(H2); polar lipid profile containing diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid; characteristic cell-wall diamino acid meso-diaminopimelic acid; whole-cell sugars galactose, xylose and ribose; absence of mycolic acids; and major fatty acids iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The results of physiological and biochemical tests allowed phenotypic differentiation of strain A1(T) from members of the genus Brevibacterium. On the basis of the results in this study, a novel species, Brevibacterium ammoniilyticum sp. nov., is proposed. The type strain is A1(T) ( = KEMC 41-098(T) = JCM 17537(T) = KACC 15558(T)).
一株革兰氏阳性、非运动性、化能有机营养型、中温好氧细菌，被命名为 A1(T)，从一家废水处理厂的污泥中分离得到。菌株 A1(T) 表现出良好的氨降解能力，并能在添加甲醇和氨的培养基上良好生长。菌株 A1(T) 在 0-11%（w/v）NaCl 下生长，最适生长温度为 20-42°C，但不能在<15 或>45°C 或 pH6-10（最适 pH 8.0-9.0）下生长。该分离株为过氧化氢酶阳性，氧化酶阴性。DNA G+C 含量为 70.7 mol%。16S rRNA 基因序列的比较分析表明，菌株 A1(T) 在短杆菌属中形成一个独特的系统发育谱系，与短杆菌属短杆菌 NCDO 2048(T)（96.9%）、短杆菌属速生短杆菌 KMM 3637(T)（96.9%）和短杆菌属血红色短杆菌 CF63(T)（96.4%）的序列相似性最高。DNA-DNA 杂交显示，该分离株与其最接近的系统发育亲缘关系之间的 DNA-DNA 相关性<43%。菌株 A1(T) 与短杆菌属的亲缘关系得到了化学分类数据的支持：主要醌类为 MK-7(H2)；极性脂谱含有双磷脂酰甘油、磷脂酰甘油和一种未知的糖脂；特征细胞壁二氨基庚二酸为 meso-二氨基庚二酸；全细胞糖为半乳糖、木糖和核糖；不存在分枝菌酸；主要脂肪酸为 iso-C15:0、anteiso-C15:0 和 anteiso-C17:0。生理生化试验结果使菌株 A1(T) 能够与短杆菌属的成员区分开来。基于本研究的结果，提出了一个新的物种，即氨解短杆菌 Brevibacterium ammoniilyticum sp. nov.。该模式株为 A1(T)（=KEMC 41-098(T)=JCM 17537(T)=KACC 15558(T)）。
