用于脑组织中神经肽和脂质质谱成像的NIMS和MALDI平台的比较。
Comparison of NIMS and MALDI platforms for neuropeptide and lipid mass spectrometric imaging in brain tissue.
作者信息
Sturm Robert M, Greer Tyler, Chen Ruibing, Hensen Broderick, Li Lingjun
机构信息
Department of Chemistry, University of Wisconsin-Madison.
出版信息
Anal Methods. 2013;5(6):1623-1628. doi: 10.1039/C3AY26067D. Epub 2013 Jan 29.
Abstract
Nanostructure-initiator mass spectrometry (NIMS) is a recently developed matrix-free laser desorption/ionization technique that has shown promise for peptide analyses. It is also useful in mass spectrometric imaging (MSI) studies of small molecule drugs, metabolites, and lipids, minimizing analyte diffusion caused by matrix application. In this study, NIMS and matrix-assisted laser desorption/ionization (MALDI) MSI of a crustacean model organism brain were compared. MALDI was found to perform better than NIMS in these neuropeptide imaging experiments. Twelve neuropeptides were identified in MALDI MSI experiments whereas none were identified in NIMS MSI experiments. In addition, lipid profiles were compared using each ionization method. Both techniques provided similar lipid profiles in the range 700 - 900.
摘要
纳米结构引发剂质谱法（NIMS）是一种最近开发的无基质激光解吸/电离技术，已显示出在肽分析方面的应用前景。它在小分子药物、代谢物和脂质的质谱成像（MSI）研究中也很有用，可将基质应用引起的分析物扩散降至最低。在本研究中，比较了甲壳类模式生物大脑的NIMS和基质辅助激光解吸/电离（MALDI）MSI。在这些神经肽成像实验中，发现MALDI的表现优于NIMS。在MALDI MSI实验中鉴定出12种神经肽，而在NIMS MSI实验中未鉴定出任何神经肽。此外，使用每种电离方法比较了脂质谱。两种技术在700 - 900范围内提供了相似的脂质谱。
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