Department of Biology, Massachussetts Institute of Technology, Cambridge, Massachussetts, United States of America.
PLoS One. 2013;8(3):e59450. doi: 10.1371/journal.pone.0059450. Epub 2013 Mar 27.
Recent analysis of genomic signatures in mammals, flies, and worms indicates that functional translational stop codon readthrough is considerably more abundant in metazoa than previously recognized, but this analysis provides only limited clues about the function or mechanism of readthrough. If an mRNA known to be read through in one species is also read through in another, perhaps these questions can be studied in a simpler setting. With this end in mind, we have investigated whether some of the readthrough genes in human, fly, and worm also exhibit readthrough when expressed in S. cerevisiae. We found that readthrough was highest in a gene with a post-stop hexamer known to trigger readthrough, while other metazoan readthrough genes exhibit borderline readthrough in S. cerevisiae.
最近对哺乳动物、果蝇和线虫的基因组特征分析表明，功能翻译性终止密码子通读比以前认为的更为普遍，但这种分析仅提供了关于通读功能或机制的有限线索。如果在一种物种中已知的 mRNA 是通读的，那么在另一种物种中也可能是通读的，也许这些问题可以在更简单的环境中进行研究。基于此目的，我们研究了在酿酒酵母中表达时，人类、果蝇和线虫中的一些通读基因是否也发生通读。我们发现，在一个已知具有触发通读的终止后六聚体的基因中，通读率最高，而其他后生动物通读基因在酿酒酵母中则表现出边缘通读。
