培养基成分对醋酸杆菌 MTCC 2623 细胞生长和细菌纤维素生产的影响。
Effect of media components on cell growth and bacterial cellulose production from Acetobacter aceti MTCC 2623.
机构信息
Department of Biotechnology, Jaypee Institute of Information Technology, Noida, A-10, Sector-62, Noida 201307, Uttar Pradesh, India.
出版信息
Carbohydr Polym. 2013 Apr 15;94(1):12-6. doi: 10.1016/j.carbpol.2013.01.018. Epub 2013 Jan 20.
Acetobacter aceti MTCC 2623 was studied as an alternative microbial source for bacterial cellulose (BC) production. Effect of media components on cell growth rate, BC production and cellulose characteristics were studied. FTIR results showed significant variations in cellulose characteristics produced by A. aceti in different media. Results have shown the role of fermentation time on crystallinity ratio of BC in different media. Further, effect of six different media components on cell growth and BC production was studied using fractional factorial design. Citric acid was found to be the most significant media component for cell growth rate (95% confidence level, R(2)=0.95). However, direct role of these parameters on cellulose production was not established (p-value>0.05).
醋化醋杆菌 MTCC 2623 被研究作为生产细菌纤维素（BC）的替代微生物来源。研究了培养基成分对细胞生长速率、BC 生产和纤维素特性的影响。傅里叶变换红外光谱（FTIR）结果表明，醋化醋杆菌在不同培养基中生产的纤维素特性有显著差异。结果表明，发酵时间对不同培养基中 BC 结晶度比的作用。此外，使用部分因子设计研究了六种不同培养基成分对细胞生长和 BC 生产的影响。柠檬酸被发现是对细胞生长速率最有影响的培养基成分（95%置信水平，R(2)=0.95）。然而，这些参数对纤维素生产的直接作用尚未确定（p 值>0.05）。
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