Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.
Carbohydr Polym. 2013 May 15;94(2):857-65. doi: 10.1016/j.carbpol.2013.02.017. Epub 2013 Feb 18.
Glucans isolated from various mushroom and mycelia sources are interestingly being studied nowadays as a potent therapeutic agent. The present work was focused on the isolation, characterization and immunomodulatory study of a novel water soluble glucan from the pure mycelia of Pleurotus ostreatus. The extracted glucan was found to have a high molecular weight of ∼2.7 × 10(6)Da and mainly comprised of glucose, mannose and fucose in a ratio of 3:2:1 with both β and α linkages. Presence of terminal or interior glucose, mannose and fucose residues was also revealed using a high throughput miniaturized platform of lectin microarray. The heteroglucan folded into a triple helical conformation and exhibited enhanced immune cell activation and anti-tumor potential in tumor bearing mice model. Thus, potential biological functions incorporated in these glucan molecules acts in accord with its structural property and exploration of such structure-function relationship will unveil its diverse mechanism of action.
如今，从各种蘑菇和菌丝体来源中分离出的葡聚糖正作为一种有效的治疗剂受到广泛关注。本研究专注于从纯平菇菌丝体中分离、表征和研究一种新型水溶性葡聚糖。研究发现，提取的葡聚糖具有较高的分子量（约 2.7×10^6Da），主要由葡萄糖、甘露糖和岩藻糖组成，比例为 3:2:1，同时具有β和α两种键连接方式。使用高通量微型化的凝集素微阵列平台也揭示了末端或内部葡萄糖、甘露糖和岩藻糖残基的存在。该杂葡聚糖折叠成三螺旋构象，并在荷瘤小鼠模型中表现出增强的免疫细胞激活和抗肿瘤潜力。因此，这些葡聚糖分子中包含的潜在生物学功能与其结构特性一致，对这种结构-功能关系的探索将揭示其多样化的作用机制。
