Chen I-Shiung
Institute of Marine Biology, National Taiwan Ocean University , Keelung 202, Taiwan , Republic of China.
Mitochondrial DNA. 2013 Dec;24(6):680-2. doi: 10.3109/19401736.2013.773985. Epub 2013 Apr 2.
In this study, the complete mitogenome sequence of Chinese sucker Myxocyprinus asiaticus (Cypriniformes, Catostomidae) has been amplified and sequenced by employing long polymerase chain reaction method. The mitogenome, consisting of 16,636 base pairs (bp), had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes, and a noncoding control region (CR). CR of 944 bp length is located between tRNA(Pro) and tRNA(Phe). The overall base composition of M. asiaticus is 28.62% for A, 27.90% for C, 24.8% for T, and 18.70% for G, with a slight AT bias of 53.40%. The complete mitogenome may provide important DNA molecular data for further phylogenetic analyses for higher taxa of teleost fishes especially for the members of Cypriniformes.
在本研究中,采用长聚合酶链反应方法扩增并测序了中华倒刺鲃(鲤形目,胭脂鱼科)的完整线粒体基因组序列。该线粒体基因组由16636个碱基对(bp)组成,具有典型的脊椎动物线粒体基因排列,包括13个蛋白质编码基因、22个转运RNA、2个核糖体RNA基因和一个非编码控制区(CR)。长度为944 bp的CR位于tRNA(Pro)和tRNA(Phe)之间。中华倒刺鲃的总体碱基组成为:A占28.62%,C占27.90%,T占24.8%,G占18.70%,AT含量略高,为53.40%。完整的线粒体基因组可能为硬骨鱼类尤其是鲤形目成员的高级分类群的进一步系统发育分析提供重要的DNA分子数据。