Han Meng, Wang Chun-Lin, Shen Chia-Ning, Chen I-Shiung
School of Marine Science, Ningbo University , Ningbo, Zhejiang , PR China .
Mitochondrial DNA. 2015 Feb;26(1):133-4. doi: 10.3109/19401736.2013.815172. Epub 2013 Jul 31.
In this study, the complete mitogenome sequence of half-spined barbel Acrossocheilus hemispinus has been amplified and sequenced by employing long polymerase chain reaction method. The mitogenome, consisting of 16,590 base pairs (bp), had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a noncoding control region (CR). CR of 940 bp length is located between tRNA(Pro) and tRNA(Phe). The overall base composition of A. hemispinus is 31.2% for A, 28.2% for C, 24.4% for T and 15.9 % for G, with a slight AT bias of 55.6%. The complete mitogenome may provide rather essential and important DNA molecular data for further phylogenetic analysis for not only congeneric species but also higher different taxa of Cyprinid fishes.
在本研究中,采用长聚合酶链反应方法扩增并测序了半刺光唇鱼(Acrossocheilus hemispinus)的完整线粒体基因组序列。该线粒体基因组由16,590个碱基对(bp)组成,具有典型的脊椎动物线粒体基因排列,包括13个蛋白质编码基因、22个转运RNA、2个核糖体RNA基因和一个非编码控制区(CR)。长度为940 bp的CR位于tRNA(Pro)和tRNA(Phe)之间。半刺光唇鱼的总体碱基组成为:A占31.2%,C占28.2%,T占24.4%,G占15.9%,AT偏倚率为55.6%。完整的线粒体基因组可能为鲤科鱼类的同属物种以及更高分类阶元的进一步系统发育分析提供相当重要的DNA分子数据。