Zheng Yunya, Feng Linxiao, Liu Haoyu, Song Riyu, Xu Shengyong, Shi Huilai, Gao Tianxiang
Fisheries College, Zhejiang Ocean University, Zhoushan, Zhejiang, China.
Zhejiang Province Key Lab of Mariculture and Enhancement, Marine Fisheries Research Institute of Zhejiang, Zhoushan, Zhejiang, China.
Mitochondrial DNA B Resour. 2020 Jul 14;5(3):2807-2808. doi: 10.1080/23802359.2020.1788463.
In this study, we obtained the complete mitochondrial genome of using whole genome sequencing. With the exception for control region, this mitochondrial genome, consisting of 16,355 base pairs (bp), contains 13 protein-coding genes (PCGs), 2 ribosomal RNAs (rRNAs), and 21 transfer RNAs (tRNAs). This mitochondrial genome also lacks a tRNA-Pro gene after tRNA-Thr gene. The overall base composition shows 25.45% of T, 29.73% of C, 28.68% of A and 16.14% of G, with a slight A + T rich feature (54.13%). Sanger sequencing is needed to confirm the accuracy of control region, as well as the lack of tRNA-Pro gene. The mitogenome data provides useful genetic markers for the studies on the molecular identification, population genetics, phylogenetic analysis and conservation genetics.
在本研究中,我们通过全基因组测序获得了[物种名称]的完整线粒体基因组。除控制区外,该线粒体基因组由16355个碱基对(bp)组成,包含13个蛋白质编码基因(PCGs)、2个核糖体RNA(rRNAs)和21个转运RNA(tRNAs)。该线粒体基因组在tRNA-Thr基因之后还缺少一个tRNA-Pro基因。总体碱基组成显示,胸腺嘧啶(T)占25.45%,胞嘧啶(C)占29.73%,腺嘌呤(A)占28.68%,鸟嘌呤(G)占16.14%,具有轻微的A+T富集特征(54.13%)。需要进行桑格测序以确认控制区的准确性以及tRNA-Pro基因的缺失。线粒体基因组数据为分子鉴定、群体遗传学、系统发育分析和保护遗传学研究提供了有用 的遗传标记。