Institute of Systematic Biology, Daugavpils University, Vienības 13, Daugavpils LV-5401, Latvia.
J Biotechnol. 2013 May 20;165(2):127-32. doi: 10.1016/j.jbiotec.2013.03.011. Epub 2013 Mar 29.
We demonstrate a method for direct delivery of metal nanoparticles to flax calli and regenerant cells by vacuum deposition of metal nanolayers on powdered hormone followed by dispersal of the combined hormone-metal in medium. The penetration and location of the gold (AuNPs) and silver (AgNPs) nanoparticles in calli and in plant regenerants were confirmed by optical absorption spectroscopy and scanning electron microscopy. We detected a significant effect of the AuNPs and AgNPs on the regeneration type of flax calli.
我们展示了一种通过将金属纳米层真空沉积在粉末状激素上，然后将结合了激素和金属的粉末分散在培养基中，将金属纳米颗粒直接递送到亚麻愈伤组织和再生细胞中的方法。通过光学吸收光谱和扫描电子显微镜证实了金（AuNPs）和银（AgNPs）纳米颗粒在愈伤组织和植物再生体中的穿透和位置。我们检测到 AuNPs 和 AgNPs 对亚麻愈伤组织的再生类型有显著影响。
