Joshi Shubham, Dar Aqib I, Acharya Amitabha, Joshi Rohit
Division of Biotechnology, CSIR-Institute of Himalayan Bioresource Technology (CSIR-IHBT), Palampur 176061, Himachal Pradesh, India.
Academy of Scientific and Innovative Research (AcSIR), CSIR-HRDC Campus, Ghaziabad 201002, Uttar Pradesh, India.
Antioxidants (Basel). 2022 Sep 30;11(10):1962. doi: 10.3390/antiox11101962.
is a critically endangered medicinal plant and endemic to the Himalayas, having high commercial demand globally. The accumulation of various secondary metabolites in its shoots and roots with antioxidant potential are well-documented in traditional as well as modern medicine systems. In the present study, we first attempted to investigate the impact of citrate (-ve charge, 11.1 ± 1.9 nm) and CTAB (+ve charge, 19.5 ± 3.2 nm) coated gold nanoparticles (AuNPs) on the in vitro proliferation and antioxidant activities of . Both the nanoparticles differentially affected the morphological and biochemical parameters, chlorophyll content, internal hormone concentration, and antioxidant activities in a concentration-dependent (10-100 µM) manner. Vigorous shooting was observed in half strength MS medium supplemented with IAA (1 mg/L) with 60 µM citrate-AuNPs (46.4 ± 3.7 mm) and 40 µM CTAB-AuNPs (42.2 ± 3.2 mm). Similarly, the maximum number of roots (5.00 ± 0.67 and 5.33 ± 0.58) and root length (29.9 ± 1.5 mm and 27.3 ± 4.8 mm) was reported in half-strength MS medium with IAA (1 mg/L) supplemented with 60 µM citrate-AuNPs and 40 µM CTAB-AuNPs, respectively. In addition, plants growing on MS medium supplemented with 60 µM citrate-AuNPs and 40 µM CTAB-AuNPs showed significantly enhanced photosynthetic pigments (chlorophyll a and b, carotenoids, and total chlorophyll), internal hormone concentration (GA3, IAA, and ABA), and antioxidant activities (total phenolics, flavonoids, DPPH, and SOD enzyme activity). Moreover, the transcript analysis of , , , , , , , and further confirmed the role of 60 µM citrate-AuNPs and 40 µM CTAB-AuNPs in the improvement in the growth and antioxidant activities of Bearing in mind the urgent requirements of the effective conservation measures of this endangered species, the present findings suggest the elicitation of citrate-AuNPs and CTAB-AuNPs would significantly improve the potential applications of in the medicinal plant-based industry.
是一种极度濒危的药用植物，为喜马拉雅地区特有，在全球具有很高的商业需求。其茎和根中积累的具有抗氧化潜力的各种次生代谢产物，在传统医学和现代医学体系中都有充分记载。在本研究中，我们首次尝试研究柠檬酸盐（带负电荷，11.1±1.9纳米）和十六烷基三甲基溴化铵（带正电荷，19.5±3.2纳米）包覆的金纳米颗粒（AuNPs）对[植物名称未给出]体外增殖和抗氧化活性的影响。两种纳米颗粒均以浓度依赖（10 - 100微摩尔）的方式对形态和生化参数、叶绿素含量、内源激素浓度及抗氧化活性产生不同影响。在添加了IAA（1毫克/升）的半强度MS培养基中，添加60微摩尔柠檬酸盐 - AuNPs（46.4±3.7毫米）和40微摩尔十六烷基三甲基溴化铵 - AuNPs（42.2±3.2毫米）时观察到旺盛的茎生长。同样，在添加了IAA（1毫克/升）的半强度MS培养基中，分别添加60微摩尔柠檬酸盐 - AuNPs和40微摩尔十六烷基三甲基溴化铵 - AuNPs时，报道的根的最大数量（分别为5.00±0.67和5.33±0.58）和根长（分别为29.9±1.5毫米和27.3±4.8毫米）。此外，在添加60微摩尔柠檬酸盐 - AuNPs和40微摩尔十六烷基三甲基溴化铵 - AuNPs的MS培养基上生长的植物，其光合色素（叶绿素a和b、类胡萝卜素及总叶绿素）、内源激素浓度（赤霉素3、吲哚乙酸和脱落酸）及抗氧化活性（总酚、类黄酮、二苯基苦味酰基自由基和超氧化物歧化酶活性）均显著增强。此外，对[相关基因未给出]的转录分析进一步证实了60微摩尔柠檬酸盐 - AuNPs和40微摩尔十六烷基三甲基溴化铵 - AuNPs在改善[植物名称未给出]生长和抗氧化活性方面的作用。考虑到对该濒危物种进行有效保护措施的迫切需求，目前的研究结果表明，柠檬酸盐 - AuNPs和十六烷基三甲基溴化铵 - AuNPs的诱导将显著改善[植物名称未给出]在药用植物产业中的潜在应用。
