Kokina Inese, Mickeviča Ilona, Jermaļonoka Marija, Bankovska Linda, Gerbreders Vjačeslavs, Ogurcovs Andrejs, Jahundoviča Inese
Department of Biotechnology, Daugavpils University, Institute of Life Sciences and Technology, Laboratory of Genomics and Biotechnology, Parades Street 1A, Daugavpils LV-5401, Latvia.
Department of Technology, Daugavpils University, Institute of Life Sciences and Technology, G. Liberts' Center of Innovative Microscopy, Parades street 1A, Daugavpils LV-5401, Latvia.
Int J Genomics. 2017;2017:1676874. doi: 10.1155/2017/1676874. Epub 2017 Feb 23.
Nanoparticles influence on genome is investigated worldwide. The appearance of somaclonal variation is a cause of great concern for any micropropagation system. Somaclonal variation describes the tissue-culture-induced phenotypic and genotypic variations. This paper shows the results of somaclonal variation in two resistance genes pectin methylesterase and Mlo-like protein in all tissue culture development stages, as donor plant, calluses, and regenerants of induced by gold and silver nanoparticles. In this paper, it was essential to obtain DNA material from all tissue culture development stages from one donor plant to record changes in each nucleotide sequence. Gene region specific primers were developed for resistance genes such as and to define the genetic variability in tissue culture of . In recent years, utilization of gold and silver nanoparticles in tissue culture is increased and the mechanisms of changes in genome induced by nanoparticles still remain unclear. Obtained data show the somaclonal variation increase in calluses obtained from one donor plant and grown on medium supplemented by gold nanoparticles ( 14.68 ± 0.98; 2.07 ± 0.87) or silver nanoparticles ( 12.01 ± 0.43; 10.04 ± 0.46) and decrease in regenerants. Morphological parameters of calluses showed a number of differences between each investigated culture group.
全世界都在研究纳米颗粒对基因组的影响。体细胞克隆变异的出现是任何微繁殖系统都极为关注的一个原因。体细胞克隆变异描述了组织培养诱导的表型和基因型变异。本文展示了在金纳米颗粒和银纳米颗粒诱导的所有组织培养发育阶段（作为供体植物、愈伤组织和再生植株）中，两个抗性基因果胶甲酯酶和Mlo样蛋白的体细胞克隆变异结果。在本文中，从同一供体植物的所有组织培养发育阶段获取DNA材料以记录每个核苷酸序列的变化至关重要。针对抗性基因开发了基因区域特异性引物，如……以确定……组织培养中的遗传变异性。近年来，金纳米颗粒和银纳米颗粒在组织培养中的应用有所增加，而纳米颗粒诱导基因组变化的机制仍不清楚。获得的数据表明，从同一供体植物获得并在添加金纳米颗粒（……）或银纳米颗粒（……）的培养基上生长的愈伤组织中体细胞克隆变异增加，而在再生植株中则减少。愈伤组织的形态学参数在每个研究的培养组之间显示出许多差异。
