Liu Ximeng, Harada Shuko
Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA.
Curr Protoc Mol Biol. 2013 Apr;Chapter 2:Unit2.14. doi: 10.1002/0471142727.mb0214s102.
DNA isolation is a fundamental initial step for molecular genetic studies. Two quite different methodologies are described in this unit: silica spin column and phenol/chloroform extraction. Currently, the most commonly used technique is the silica-based spin column, although phenol/chloroform extraction is still widely used. This unit also presents basic procedures for extraction of DNA from both fresh tissues and formalin-fixed, paraffin-embedded (FFPE) tissue samples.
DNA提取是分子遗传学研究的基本初始步骤。本单元介绍了两种截然不同的方法：硅胶离心柱法和酚/氯仿提取法。目前，最常用的技术是基于硅胶的离心柱法，不过酚/氯仿提取法仍被广泛使用。本单元还介绍了从新鲜组织以及福尔马林固定、石蜡包埋（FFPE）组织样本中提取DNA的基本步骤。
