利用水杨酸包覆的磁性纳米颗粒一步法提取基因组 DNA。
One-stop genomic DNA extraction by salicylic acid-coated magnetic nanoparticles.
机构信息
Bindley Bioscience Center, Purdue University, West Lafayette, IN 47906, USA; Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47906, USA.
出版信息
Anal Biochem. 2013 Nov 15;442(2):249-52. doi: 10.1016/j.ab.2013.07.030. Epub 2013 Jul 31.
Abstract
Salicylic acid-coated magnetic nanoparticles were prepared via a modified one-step synthesis and used for a one-stop extraction of genomic DNA from mammalian cells. The synthesized magnetic particles were used for magnetic separation of cells from the media by nonspecific binding of the particles as well as extraction of genomic DNA from the lysate. The quantity and quality were confirmed by agarose gel electrophoresis and polymerase chain reaction. The entire process of extraction and isolation can be completed within 30 min. Compared with traditional methods based on centrifugation and filtration, the established method is fast, simple, reliable, and environmentally friendly.
摘要
水杨酸包覆的磁性纳米粒子通过改良的一步合成法制备，并用于从哺乳动物细胞中一步提取基因组 DNA。合成的磁性颗粒通过颗粒的非特异性结合以及从裂解物中提取基因组 DNA 用于细胞与培养基的磁性分离。通过琼脂糖凝胶电泳和聚合酶链反应确认数量和质量。提取和分离的整个过程可以在 30 分钟内完成。与基于离心和过滤的传统方法相比，所建立的方法快速、简单、可靠且环保。
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