智利安第斯高地盐沼嗜盐菌去除亚砷酸盐的动力学
Kinetics of arsenite removal by halobacteria from a highland Andean Chilean Salar.
作者信息
Paula Díaz-Palma, Gleny Alfaro, Martha Hengst, Patricia Pozo, Susana Stegen, Fabrizio Queirolo, Gonzalo Rojo, Pedro Silva, Diana Arias, Karem Gallardo, Carlos Contreras-Ortega
机构信息
Departamento de Química, Facultad de Ciencias, Universidad Católica del Norte, P,O,1280, Antofagasta, Chile.
出版信息
Aquat Biosyst. 2013 Apr 1;9(1):8. doi: 10.1186/2046-9063-9-8.
BACKGROUND
The purpose of this study was to identify arsenite-oxidizing halobacteria in samples obtained from Salar de Punta Negra, II Region of Chile. Seven bacterial isolates, numbered as isolates I to VII, grown in a culture medium with 100 ppm as NaAsO2 (As (III)) were tested. Bacterial growth kinetics and the percent of arsenite removal (PAR) were performed simultaneously with the detection of an arsenite oxidase enzyme through Dot Blot analysis.
RESULTS
An arsenite oxidase enzyme was detected in all isolates, expressed constitutively after 10 generations grown in the absence of As (III). Bacterial growth kinetics and corresponding PAR values showed significant fluctuations over time. PARs close to 100% were shown by isolates V, VI, and VII, at different times of the bacterial growth phase; while isolate II showed PAR values around 40%, remaining constant over time.
CONCLUSION
Halobacteria from Salar de Punta Negra showed promising properties as arsenite removers under control conditions, incubation time being a critical parameter.
背景
本研究的目的是在从智利第二大区蓬塔内格拉盐沼采集的样本中鉴定亚砷酸盐氧化嗜盐菌。对在含有100 ppm 亚砷酸钠（As(III)）的培养基中生长的7株细菌分离株（编号为分离株I至VII）进行了测试。在通过斑点印迹分析检测亚砷酸盐氧化酶的同时，进行了细菌生长动力学和亚砷酸盐去除率（PAR）的测定。
结果
在所有分离株中均检测到亚砷酸盐氧化酶，在无As(III)的情况下生长10代后组成型表达。细菌生长动力学和相应的PAR值随时间显示出显著波动。分离株V、VI和VII在细菌生长阶段的不同时间显示出接近100%的PAR；而分离株II的PAR值约为40%，随时间保持恒定。
结论
在可控条件下，蓬塔内格拉盐沼的嗜盐菌作为亚砷酸盐去除剂显示出良好的特性，培养时间是一个关键参数。
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