Thapar M A, Parr E L, Parr M B
Department of Anatomy, School of Medicine, Southern Illinois University, Carbondale 62901-6503.
Immunology. 1990 May;70(1):121-5.
Intravaginal immunization causes IgA responses in vaginal fluid, but so far lymphoid nodules in mouse vaginal mucosa have not been detected. The present study was therefore designed to test the hypothesis that IgA responses in the female reproductive tract may be generated in the regional iliac lymph nodes. Two, non-mucosal sites were identified in the female mouse pelvis, the subserous and presacral spaces, from which lymph drains mainly to the iliac nodes. Immunization at these pelvic sites with horse ferritin adsorbed to aluminum hydroxide (AH) caused much higher IgA and IgG titres in vaginal fluid than intravaginal immunization; moreover, the pelvic immunizations caused significantly higher and better sustained IgA titres in vaginal fluid than subcutaneous immunization near the scapulae or in the perineum, while IgG titres in vaginal fluid were similar in these groups. Additional mice were immunized with ferritin subcutaneously near the scapulae or in the presacral pelvic space using dimethyl dioctadecyl ammonium bromide (DDA), AH plus muramyl dipeptide, or the Ribi adjuvant system as adjuvants. Pelvic immunization caused higher IgA titres in vaginal fluid than subcutaneous immunization in each case. The IgA response stimulated by DDA was similar to that produced by AH but higher than the responses caused by the other two adjuvants, while IgG titres were similar with all four adjuvants in both sites. The results suggest that non-mucosal, pelvic immunization is particularly effective in stimulating IgA responses in the female reproductive tract. The observation is consistent with the possibility that the iliac lymph nodes may play a role in the development of IgA responses in the reproductive tract.
阴道内免疫可引起阴道液中的IgA反应，但迄今为止尚未在小鼠阴道黏膜中检测到淋巴小结。因此，本研究旨在验证以下假说：女性生殖道中的IgA反应可能在髂淋巴结中产生。在雌性小鼠骨盆中确定了两个非黏膜部位，即浆膜下间隙和骶前间隙，从这些部位引流的淋巴液主要流向髂淋巴结。用吸附于氢氧化铝（AH）的马铁蛋白在这些骨盆部位进行免疫，比阴道内免疫在阴道液中引起更高的IgA和IgG滴度；此外，骨盆免疫在阴道液中引起的IgA滴度明显高于肩胛附近或会阴皮下免疫，且维持时间更长，而这些组阴道液中的IgG滴度相似。另外，用二甲基二十八烷基溴化铵（DDA）、AH加胞壁酰二肽或Ribi佐剂系统作为佐剂，在肩胛附近或骶前骨盆间隙对小鼠进行皮下铁蛋白免疫。在每种情况下，骨盆免疫在阴道液中引起的IgA滴度均高于皮下免疫。DDA刺激产生的IgA反应与AH产生的相似，但高于其他两种佐剂引起的反应，而在两个部位，所有四种佐剂产生的IgG滴度相似。结果表明，非黏膜性骨盆免疫在刺激女性生殖道中的IgA反应方面特别有效。这一观察结果与髂淋巴结可能在生殖道IgA反应的发生中起作用的可能性相一致。
