Spencer J, Gyure L A, Hall J G
Immunology. 1983 Apr;48(4):687-93.
Eight weeks after rats had had their mesenteric lymph nodes (MLN) removed surgically, they were found to be still able to generate substantial titres of biliary IgA-antibodies after antigens were injected into their Peyer's patches. This suggested that systemically significant IgA production could be induced in extra-abdominal lymphoid tissue. It was found that the intrathoracic lymph nodes (ITLN) were an important source of IgA production. These nodes could be stimulated to produce biliary antibody by introducing antigen either into the peritoneal cavity or directly into the thorax. Cells forming IgA were identified in the ITLNs by haemolytic plaque assays and immunoperoxidase techniques. In spite of this, immunoblasts obtained from the ITLNs, and labelled with 125IUdR did not localize in the gut after i.v. injection to anywhere near the extent that immunoblasts from the MLN did. Instead they seemed to have a predilection for localizing in the lungs.
大鼠肠系膜淋巴结（MLN）被手术切除八周后，研究人员发现，在将抗原注入派尔集合淋巴结后，它们仍能够产生大量的胆汁IgA抗体。这表明，在腹外淋巴组织中可诱导产生具有全身意义的IgA。研究发现，胸内淋巴结（ITLN）是IgA产生的重要来源。通过将抗原引入腹腔或直接引入胸腔，可刺激这些淋巴结产生胆汁抗体。通过溶血空斑试验和免疫过氧化物酶技术在胸内淋巴结中鉴定出形成IgA的细胞。尽管如此，从胸内淋巴结获得并用125I UdR标记的免疫母细胞在静脉注射后，并没有像肠系膜淋巴结的免疫母细胞那样在肠道中大量定位。相反，它们似乎更倾向于在肺部定位。
