State Key Laboratory of Biotherapy, West China Hospital, West China Medical School, Sichuan University, Chengdu, China.
Int J Biol Macromol. 2013 Jul;58:79-86. doi: 10.1016/j.ijbiomac.2013.03.061. Epub 2013 Mar 30.
In this work, a new kind of biodegradable poly(pluronic-co-L-lactide) (Pluronic-PLLA copolymers) was successfully prepared by melt-polycondensation method from L-lactide, Pluronic and isophorone diisocyanate (IPDI). The obtained copolymers were characterized by (1)H NMR, FT-IR, X-ray, and TGA/DTA. Meanwhile, three-dimensional (3-D) porous scaffolds based on Pluronic-PLLA were prepared by the electrospinning method, the factors of concentration, flow rate and voltage that influence the formation of the Pluronic-PLLA nanofibers were studied and the structure of Pluronic-PLLA nanofibers were investigated by scanning electron microscopy (SEM). MTT results revealed that the Pluronic-PLLA scaffolds had good biocompatibility and nontoxicity. Morphological study using fluorescence micrographs and scanning electron microscopy showed that in vitro osteoblast cell culture demonstrated the electrospun Pluronic-PLLA composite scaffolds could provide a suitable environment for good cell attachment. These results suggested that such Pluronic-PLLA nanofibers membranes might have prospective applications in tissue engineering field.
在这项工作中，通过熔融缩聚法成功地从 L-丙交酯、Pluronic 和异佛尔酮二异氰酸酯 (IPDI) 制备了一种新型可生物降解的聚（Pluronic-co-L-丙交酯）（Pluronic-PLLA 共聚物）。通过 1H NMR、FT-IR、X 射线和 TGA/DTA 对得到的共聚物进行了表征。同时，通过静电纺丝法制备了基于 Pluronic-PLLA 的三维（3-D）多孔支架，研究了浓度、流速和电压等因素对 Pluronic-PLLA 纳米纤维形成的影响，并通过扫描电子显微镜（SEM）研究了 Pluronic-PLLA 纳米纤维的结构。MTT 结果表明 Pluronic-PLLA 支架具有良好的生物相容性和低毒性。荧光显微镜和扫描电子显微镜的形态学研究表明，体外成骨细胞培养表明，静电纺丝 Pluronic-PLLA 复合支架可为良好的细胞附着提供合适的环境。这些结果表明，这种 Pluronic-PLLA 纳米纤维膜可能在组织工程领域具有广阔的应用前景。
