Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China; University of Chinese Academy of Sciences, Beijing 100039, China.
Acta Biomater. 2014 Dec;10(12):5074-5080. doi: 10.1016/j.actbio.2014.08.036. Epub 2014 Sep 6.
Blends of aniline pentamer-graft-gelatin (AP-g-GA) and poly(l-lactide) (PLLA) were electrospun to prepare uniform nanofibers as biomimetic scaffolds. The nanofibers exhibited good electroactivity, thermal stability and biodegradability. The biocompatibility of the nanofibers in vitro was evaluated by the adhesion and proliferation of mouse preosteoblastic MC3T3-E1 cells. The cellular elongation was significantly greater on electroactive AP-g-GA/PLLA nanofibers than on PLLA nanofibers. Moreover, the AP-g-GA/PLLA nanofibers stimulated by an electrical pulsed signal could promote the differentiation of MC3T3-E1 cells compared with pure PLLA nanofibers. Our results demonstrated that the biodegradable and electroactive AP-g-GA/PLLA nanofibers had potential application in vivo as bone repair scaffold materials in tissue engineering.
苯胺五聚体接枝明胶（AP-g-GA）和聚（L-乳酸）（PLLA）的混合物被电纺成均匀的纳米纤维，用作仿生支架。纳米纤维表现出良好的电活性、热稳定性和生物降解性。通过小鼠前成骨细胞 MC3T3-E1 细胞的黏附和增殖来评估纳米纤维的体外生物相容性。与 PLLA 纳米纤维相比，在具有电活性的 AP-g-GA/PLLA 纳米纤维上细胞的伸长明显更大。此外，与纯 PLLA 纳米纤维相比，受电脉冲信号刺激的 AP-g-GA/PLLA 纳米纤维可促进 MC3T3-E1 细胞的分化。我们的结果表明，可生物降解和具有电活性的 AP-g-GA/PLLA 纳米纤维具有作为组织工程中骨修复支架材料的体内应用潜力。
