a Department of Pharmaceutics, Faculty of Pharmacy , Tehran University of Medical Sciences, International Campus (TUMS-IC) , Tehran , Iran.
b School of Pharmacy, College of Health Sciences , Addis Ababa University , Addis Ababa , Ethiopia.
Artif Cells Nanomed Biotechnol. 2018 Sep;46(6):1274-1281. doi: 10.1080/21691401.2017.1367928. Epub 2017 Aug 24.
Poly-L-lactic acid (PLLA) nano fibrous scaffolds prepared by electrospinning technology have been used widely in tissue engineering applications. However, PLLA scaffolds are hydrophobic in nature, moreover the fibrous porous structure produced by electrospinning makes the scaffolds even more hydrophobic which generally limits cell attachment and proliferation. Polymer blending is one of the several efforts used so far to enhance hydrophilicity and recognized as an easy cost-effective approach for the manipulation physiochemical properties of polymeric biomaterials. Pluronic block copolymers containing hydrophilic poly(ethylene oxide) (PEO) blocks and hydrophobic poly(propylene oxide) (PPO) blocks are arranged in triblock structure: PEO-PPO-PEO. It is commonly used recently to blend hydrophobic polymers to enhance hydrophilicity for pharmaceutical and tissue engineering applications. In this study, novel pluronic P123 blend PLLA electrospun nanofibre scaffolds with improved hydrophilicity and biological properties were fabricated. The surface morphology and surface chemistry of the nanofibre scaffolds were characterized by scanning electron microscope (SEM) and FTIR analyses. Surface hydrophilicity and change in mechanical properties were studied. The ability of the scaffolds to support the attachment, and proliferation and differentiation of human adipose tissue derived MSCs, were evaluated generally. The fabricated scaffolds have completely improved, hydrophilicity, similar osteogenic differentiation potential with plasma-treated PLLA nanofibre scaffold, and hence P123 blend PLLA electrospun nanofibre scaffolds are a very good and cost effective choice as a scaffold for bone tissue engineering application.
聚 L-乳酸（PLLA）纳米纤维支架通过静电纺丝技术制备，已广泛应用于组织工程应用中。然而，PLLA 支架本质上是疏水性的，而且静电纺丝产生的纤维多孔结构使支架更加疏水性，这通常限制了细胞的附着和增殖。聚合物共混是迄今为止提高亲水性的几种努力之一，被认为是改变聚合物生物材料理化性能的一种简单且具有成本效益的方法。含有亲水性聚（环氧乙烷）（PEO）嵌段和疏水性聚（环氧丙烷）（PPO）嵌段的 Pluronic 嵌段共聚物排列在三嵌段结构中：PEO-PPO-PEO。它最近常用于共混疏水性聚合物以提高亲水性，用于制药和组织工程应用。在这项研究中，制备了具有改善的亲水性和生物性能的新型 Pluronic P123 共混 PLLA 静电纺纳米纤维支架。通过扫描电子显微镜（SEM）和傅里叶变换红外（FTIR）分析对纳米纤维支架的表面形貌和表面化学进行了表征。研究了表面亲水性和机械性能的变化。评估了支架支持人脂肪组织来源间充质干细胞附着、增殖和分化的能力。所制备的支架的亲水性得到了完全改善，具有与等离子体处理 PLLA 纳米纤维支架相似的成骨分化潜力，因此 P123 共混 PLLA 静电纺纳米纤维支架是骨组织工程应用中非常好且具有成本效益的支架选择。
