Novel P, Fernández-Trujillo M A, Gallardo-Gálvez J B, Cano I, Manchado M, Buonocore F, Randelli E, Scapigliati G, Alvarez M C, Béjar J
Department of Genetics, Faculty of Sciences, University of Málaga, 29071 Málaga, Spain.
Vet Immunol Immunopathol. 2013 Jun 15;153(3-4):240-8. doi: 10.1016/j.vetimm.2013.03.003. Epub 2013 Mar 13.
Mx proteins are key components of the antiviral state triggered by interferon type I in response to viral infections. In this study, two different Mx genes have been identified in European sea bass (Dicentrarchus labrax), and their sequences were cloned and characterized. MxA cDNA consists of 1881 bp coding for a putative 626 aminoacids protein, while MxB cDNA has 1920 bp and results in a protein with 639 residues. Their corresponding genomic sequences contain 3538 bp and 5326 bp, respectively, and both present 12 exons and 11 introns. The expression patterns of the two Mx genes after an in vivo challenge with the viral nervous necrosis virus (VNNV), a serious pathogen in farmed European sea bass, have been characterized by real-time PCR. The results showed interesting differences in the transcription profile of both Mx, thus suggesting a differential role for each Mx isoform in the immune response of European sea bass to VNNV, and most likely in the general viral response of this species.
Mx蛋白是I型干扰素在病毒感染时触发的抗病毒状态的关键组成部分。在本研究中，在欧洲海鲈（Dicentrarchus labrax）中鉴定出了两种不同的Mx基因，并对其序列进行了克隆和表征。MxA cDNA由1881 bp组成，编码一个推定的626个氨基酸的蛋白质，而MxB cDNA有1920 bp，产生一个含有639个残基的蛋白质。它们相应的基因组序列分别包含3538 bp和5326 bp，且均有12个外显子和11个内含子。通过实时PCR对欧洲养殖海鲈的一种严重病原体——病毒性神经坏死病毒（VNNV）进行体内攻击后，对这两种Mx基因的表达模式进行了表征。结果显示，两种Mx的转录谱存在有趣的差异，这表明每种Mx异构体在欧洲海鲈对VNNV的免疫反应中，很可能在该物种的一般病毒反应中发挥不同的作用。
