IL28B基因分型在伊朗慢性丙型肝炎患者和健康个体中的分布

Distribution of IL28B Genotypes in Iranian Patients with Chronic Hepatitis C and Healthy Individuals.

作者信息

Sharafi Heidar, Pouryasin Ali, Alavian Seyed Moayed, Behnava Bita, Keshvari Maryam, Salimi Shima, Mehrnoush Leila, Fatemi Ahmad

机构信息

Armin Pathobiology Laboratory, Tehran, Iran ; Tehran Hepatitis Cohort (THC) Study Center, Tehran, Iran.

出版信息

Hepat Mon. 2012 Dec;12(12):e8387. doi: 10.5812/hepatmon.8387. Epub 2012 Dec 22.

BACKGROUND

IL28B polymorphism is recognized as one of the most prominent predictors of hepatitis C spontaneous and treatment-induced clearance. Interestingly, the favorable genotypes of IL28B are found to be more frequent in Asian ethnicity than Caucasian and African populations, respectively. A few studies reported that there is a mysterious association between the IL28B polymorphism and the hepatitis C virus (HCV) genotype in patients with chronic hepatitis C but they did not give any reason for this phenomenon.

OBJECTIVES

The foremost purpose of this study was to compare the distribution of IL28B genotypes between Iranian healthy individuals and patients with chronic hepatitis C.

PATIENTS AND METHODS

In this study, 921 patients with chronic hepatitis C and 142 healthy individuals were included. The IL28B rs12979860 and rs8099917 polymorphisms were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.

RESULTS

The frequency of IL28B rs12979860 CC, CT, and TT genotypes in chronic hepatitis C patients was 38%, 48.8%, and 13.2% and in healthy individuals was 43.7%, 48.6%, and 7.7%. Also, the frequency of IL28B rs8099917 TT, GT, and GG genotypes in chronic hepatitis C patients was 58.3%, 37.1%, and 4.6% and in healthy individuals was 64.1%, 32.4% and 3.5%. The differences in the distribution of IL28B rs12979860 and rs8099917 genotypes between patients with chronic hepatitis C and healthy individuals were not statistically significant. When we compared the distribution of IL28B genotypes between the healthy group and the HCV infected patients by HCV genotype, we found 9.8% higher frequency of rs12979860 CC genotype in the healthy individuals than HCV genotype 1 infected patients (P = 0.03) however there was no significant difference in the distribution of rs12979860 genotypes between the healthy and HCV genotype 3 infected groups (P = 0.46).

CONCLUSIONS

It seems that the impact of IL28B polymorphism on the spontaneous clearance of HCV genotype 1 is more prominent than HCV genotype 3 which results in the observation of higher rs12979860 C allele frequency in chronic hepatitis C patients with HCV genotype 3 than HCV genotype 1.

背景

白细胞介素28B（IL28B）基因多态性被认为是丙型肝炎自发清除和治疗诱导清除的最显著预测指标之一。有趣的是，发现IL28B的有利基因型在亚洲人种中比在白种人和非洲人群中更常见。一些研究报告称，慢性丙型肝炎患者中IL28B基因多态性与丙型肝炎病毒（HCV）基因型之间存在神秘关联，但他们没有对此现象给出任何原因。

目的

本研究的首要目的是比较伊朗健康个体与慢性丙型肝炎患者中IL28B基因型的分布。

患者和方法

本研究纳入了921例慢性丙型肝炎患者和142例健康个体。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法对IL28B rs12979860和rs8099917基因多态性进行基因分型。

结果

慢性丙型肝炎患者中IL28B rs12979860 CC、CT和TT基因型的频率分别为38%、48.8%和13.2%，健康个体中分别为43.7%、48.6%和7.7%。此外，慢性丙型肝炎患者中IL28B rs8099917 TT、GT和GG基因型的频率分别为58.3%、37.1%和4.6%，健康个体中分别为64.1%、32.4%和3.5%。慢性丙型肝炎患者与健康个体之间IL28B rs12979860和rs8099917基因型分布的差异无统计学意义。当我们按HCV基因型比较健康组与HCV感染患者之间IL28B基因型的分布时，发现健康个体中rs12979860 CC基因型的频率比HCV 1型感染患者高9.8%（P = 0.03），然而健康组与HCV 3型感染组之间rs12979860基因型的分布无显著差异（P = 0.46）。