Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
D'Or Institute for Research and Education (IDOR), Rio de Janeiro, RJ, Brazil.
Biomed Res Int. 2018 May 20;2018:2931486. doi: 10.1155/2018/2931486. eCollection 2018.
Genotype 1 of hepatitis C virus (HCV) is the most prevalent worldwide. Pegylated-interferon and ribavirin therapy is still used in the developing world but has less efficiency in this genotype. Single nucleotide polymorphisms (SNPs) rs12979860 and rs8099917 (IL28B) and rs1800896, rs1800871, and rs1800872 (IL10) are related to treatment outcome, but previous studies clustered nonresponse and relapse patients. The aim of this study is to analyze the frequency of those SNPs in HCV genotype 1 for response, nonresponse, or relapse. Patients were classified according to treatment outcome. Genomic DNA was extracted by blood samples and SNPs were defined by PCR and sequencing. Data analysis was performed with R project. The frequency of rs12979860 CC was similar among responders (0.48) and relapsers (0.46) and lower among nonresponders (0.18). The same trend was observed for rs8099917 TT. rs12979860 CC showed a protective effect for relapsers compared to nonresponders (OR = 0.25) as it occurs with responders (OR = 0.17). Haplotypes 12979860/C rs8099917/T were associated with protection against the nonresponder phenotype compared to responders (OR = 0.27) or relapsers (OR = 0.37). Frequency of rs12979860 and rs8099917 is different between relapsers and nonresponders, but similar between relapsers and responders.
丙型肝炎病毒（HCV）基因型 1 是全球最常见的类型。聚乙二醇干扰素和利巴韦林治疗在发展中国家仍在使用，但在这种基因型中效果较差。单核苷酸多态性（SNP）rs12979860 和 rs8099917（IL28B）以及 rs1800896、rs1800871 和 rs1800872（IL10）与治疗结果相关，但以前的研究将无应答和复发患者进行了聚类。本研究旨在分析 HCV 基因型 1 中这些 SNP 的频率与应答、无应答或复发的关系。根据治疗结果对患者进行分类。从血液样本中提取基因组 DNA，通过 PCR 和测序定义 SNP。使用 R 项目进行数据分析。应答者（0.48）和复发者（0.46）中 rs12979860 CC 的频率相似，无应答者（0.18）中 CC 的频率较低。rs8099917 TT 也呈现出相同的趋势。与无应答者相比，rs12979860 CC 对复发者具有保护作用（OR = 0.25），与应答者（OR = 0.17）相同。与应答者（OR = 0.27）或复发者（OR = 0.37）相比，12979860/rs8099917/T 单倍型与对无应答表型的保护作用相关。rs12979860 和 rs8099917 的频率在复发者和无应答者之间不同，但在复发者和应答者之间相似。
